Brew-Appiah Rhoda A T, Peracchi Luigi M, Sanguinet Karen A
Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6420, USA.
Plants (Basel). 2019 Jun 6;8(6):153. doi: 10.3390/plants8060153.
is an established model for monocotyledonous plants. Numerous markers intended for gene discovery and population genetics have been designed. However to date, very few indel markers with larger and easily scored length polymorphism differences, that distinguish between the two morphologically similar and highly utilized accessions, Bd21, the reference genome accession, and Bd21-3, the transformation-optimal accession, are publically available. In this study, 22 indel markers were designed and utilized to produce length polymorphism differences of 150 bp or more, for easy discrimination between Bd21 and Bd21-3. When tested on four other accessions, one case of multiallelism was observed. It was also shown that the markers could be used to determine homozygosity and heterozygosity at specific loci in a Bd21 x Bd3-1 F2 population. The work done in this study allows researchers to maintain the fidelity of Bd21 and Bd21-3 stocks for both transgenic and nontransgenic studies. It also provides markers that can be utilized in conjunction with others already available for further research on population genetics, gene discovery and gene characterization, all of which are necessary for the relevance of as a model species.
是单子叶植物的一个成熟模型。已经设计了许多用于基因发现和群体遗传学的标记。然而，迄今为止，公开可用的插入缺失标记非常少，这些标记具有更大且易于评分的长度多态性差异，可区分两种形态相似且高度常用的种质，即参考基因组种质Bd21和转化最佳种质Bd21-3。在本研究中，设计并利用了22个插入缺失标记，以产生150 bp或更长的长度多态性差异，便于区分Bd21和Bd21-3。在对其他四个种质进行测试时，观察到了一例多等位基因现象。研究还表明，这些标记可用于确定Bd21×Bd3-1 F2群体中特定位点的纯合性和杂合性。本研究开展的工作使研究人员能够在转基因和非转基因研究中保持Bd21和Bd21-3种质的纯度。它还提供了可与其他现有标记结合使用的标记，用于群体遗传学、基因发现和基因表征的进一步研究，所有这些对于作为模式物种的相关性而言都是必要的。
