Jonsson R, Klareskog L, Bäckman K, Tarkowski A
Department of Oral Pathology, University of Göteborg, Sweden.
Clin Immunol Immunopathol. 1987 Nov;45(2):235-43. doi: 10.1016/0090-1229(87)90038-9.
The expression of beta 2-microglobulin (beta 2m), class II major histocompatibility complex (MHC) antigens (HLA-DP, -DQ, -DR) and the interleukin 2 receptor (IL-2R) on resident and infiltrating cells in labial salivary glands of patients with Sjögren's syndrome (SS) was studied using an immunoperoxidase technique based on staining with monoclonal antibodies. The progression of the inflammatory process was accompanied by increasing numbers of immunocompetent cells as well as glandular epithelial cells expressing beta 2m and class II MHC antigens. Up to 60% of the infiltrating lymphocytes were DQ- and DR-positive while fewer cells stained for DP antigen. Glandular epithelium (acinar and ductal cells) stained for products of all three HLA-D subregions but with varying degrees of expression following the pattern DR greater than DP greater than DQ related to the severity of inflammation. A minor portion of the infiltrating lymphocytes expressed IL-2R. These findings emphasize the potential importance of epithelial expression of class II antigens in the local activation of T lymphocytes in salivary gland lesions of SS patients. They also indicate that differences may exist in this respect between T-cell reactions restricted by different class II MHC-encoded gene products.
运用基于单克隆抗体染色的免疫过氧化物酶技术，研究了干燥综合征（SS）患者唇腺中驻留细胞和浸润细胞上β2-微球蛋白（β2m）、Ⅱ类主要组织相容性复合体（MHC）抗原（HLA-DP、-DQ、-DR）以及白细胞介素2受体（IL-2R）的表达情况。炎症过程的进展伴随着免疫活性细胞以及表达β2m和Ⅱ类MHC抗原的腺上皮细胞数量的增加。高达60%的浸润淋巴细胞DQ和DR呈阳性，而染色显示DP抗原的细胞较少。腺上皮（腺泡细胞和导管细胞）对所有三个HLA-D亚区的产物均有染色，但随着炎症严重程度的增加，其表达程度呈DR＞DP＞DQ的模式变化。一小部分浸润淋巴细胞表达IL-2R。这些发现强调了Ⅱ类抗原在上皮细胞中的表达在SS患者唾液腺病变中T淋巴细胞局部激活方面的潜在重要性。它们还表明，受不同Ⅱ类MHC编码基因产物限制的T细胞反应在这方面可能存在差异。
