Nott J A, Moore M N
Marine Biological Association of the UK, Plymouth, Devon.
Histochem J. 1987 Jun-Jul;19(6-7):357-68. doi: 10.1007/BF01680453.
Some effects of two isomeric polycyclic aromatic hydrocarbons, anthracene and phenanthrene, on the fine structure and cytochemistry of digestive cells in the marine mussel Mytilus edulis have been investigated. The cytochemical results show that increasing concentrations of anthracene and phenanthrene have different effects on the acid labilization time for latent beta-glucuronidase which is used to measure the stability of lysosomal membranes. At the ultrastructural level the limiting membranes of secondary lysosomes appear multilayered, with discontinuities and overlaps. Under the conditions of the experiment, only phenanthrene produces changes in this configuration. Both macroautophagic and microautophagic processes occur in the control and hydrocarbon treatments, and complementary data from other studies indicate that autophagic processes are enhanced by polycyclic aromatic hydrocarbons. Phenanthrene also causes proliferation of the smooth endoplasmic reticulum in the digestive cells, although cytochemical measurements of smooth endoplasmic reticulum-associated NADPH-ferrihemoprotein reductase show that anthracene stimulates activity over a greater range of concentrations than phenanthrene. The different effects of the two isomers is taken as evidence that the molecular configuration of the compound determines its reactivity with membranes and its subsequent effect on the physiology of the cells.
研究了两种同分异构多环芳烃——蒽和菲，对海洋贻贝紫贻贝消化细胞的精细结构和细胞化学的某些影响。细胞化学结果表明，蒽和菲浓度的增加，对用于测量溶酶体膜稳定性的潜在β-葡萄糖醛酸酶的酸不稳定时间有不同影响。在超微结构水平上，次级溶酶体的界膜呈现多层结构，有间断和重叠。在实验条件下，只有菲会使这种结构发生变化。在对照和烃处理组中均发生了巨自噬和微自噬过程，其他研究的补充数据表明多环芳烃会增强自噬过程。菲还会导致消化细胞中滑面内质网增殖，尽管对滑面内质网相关的NADPH-高铁血红蛋白还原酶的细胞化学测量表明，在比菲更大的浓度范围内，蒽会刺激该酶的活性。这两种异构体的不同影响被视为化合物的分子构型决定其与膜的反应性及其对细胞生理后续影响的证据。
