组织块的骤冷：冷却速率的决定因素
Plunge-cooling of tissue blocks: determinants of cooling rates.
作者信息
Ryan K P, Purse D H
出版信息
J Microsc. 1985 Oct;140(Pt 1):47-54. doi: 10.1111/j.1365-2818.1985.tb02659.x.
Tissue blocks have been plunged into a liquid coolant and the resultant ice-crystal damage is discussed. Some blocks have been frozen without apparent ice-crystal damage; they were not treated with cryo-protectants but were mounted on streamlined foil supports which separated the specimen from the thermal mass of the support pin. Hydrated gelatin specimens of various sizes have been built around thermocouples and plunged at different speeds. Results show that cooling is relatively slow in specimens mounted on solid metal supports. In specimens mounted on foil supports cooling is related to plunge velocity in 0.25 mm diameter specimens but in 1 mm diameter specimens cooling occurs mainly after coming to rest. In 0.5 mm diameter specimens cooling is related to plunge velocity at low immersion speeds but then it decreases with increasing plunge velocity; this trend should allow monitoring of attempts to improve the efficiency of the plunge-cooling technique.
组织块已被投入液体冷却剂中，并对由此产生的冰晶损伤进行了讨论。一些组织块在冷冻时没有明显的冰晶损伤；它们没有用冷冻保护剂处理，而是安装在流线型箔片支架上，该支架将标本与支撑针的热质量隔离开来。围绕热电偶制作了各种尺寸的水合明胶标本，并以不同速度投入。结果表明，安装在固体金属支架上的标本冷却相对较慢。在安装在箔片支架上的标本中，0.25毫米直径的标本冷却与投入速度有关，但1毫米直径的标本冷却主要在静止后发生。在0.5毫米直径的标本中，低浸入速度下冷却与投入速度有关，但随后随着投入速度的增加而降低；这种趋势应该有助于监测提高投入冷却技术效率的尝试。
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