Zhang Jihui, Robinson Clive
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.
Institute for Infection and Immunity, St George's University of London, London, UK.
Methods Mol Biol. 2019;2020:185-205. doi: 10.1007/978-1-4939-9591-2_14.
House dust mites are globally significant triggers of allergic disease. Notable among their extensive repertoire of allergens are the Group 1 cysteine peptidase allergens which function as digestive enzymes in house dust mites. Compelling evidence suggests that the proteolytic activity of these molecules plays a key role in the development and maintenance of allergic diseases through the activation of innate immune mechanisms which exploit genetic predispositions to allergy. Growing interest in this area creates a requirement for high-quality purified protein, whether natural or recombinantly expressed. It has also identified these allergens as therapeutic targets for a novel approach to allergy treatment through modulation of innate immune responses. The purpose of this chapter is to describe a new method for the purification of Der p 1 and use of the protein produced in a screening assay designed for the discovery of novel inhibitors of Group 1 house dust mite allergens.
屋尘螨是全球范围内引发过敏性疾病的重要因素。在其种类繁多的过敏原中，值得注意的是1类半胱氨酸蛋白酶过敏原，它们在屋尘螨中作为消化酶发挥作用。有力证据表明，这些分子的蛋白水解活性通过激活利用过敏遗传易感性的先天免疫机制，在过敏性疾病的发生和维持中起关键作用。该领域日益增长的兴趣产生了对高质量纯化蛋白的需求，无论是天然的还是重组表达的。这也将这些过敏原确定为通过调节先天免疫反应来治疗过敏的新方法的治疗靶点。本章的目的是描述一种纯化Der p 1的新方法，以及将所产生的蛋白用于一种筛选试验，该试验旨在发现1类屋尘螨过敏原的新型抑制剂。
