He Zhongqi, Sleighter Rachel L, Hatcher Patrick G, Liu Shasha, Wu Fengchang, Zou Haixuan, Olanya O Modesto
Southern Regional Research Center, USDA Agricultural Research Service, 1100 Robert E. Lee Blvd, New Orleans, LA, 70124, USA.
Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, VA, 23529, USA.
J Mass Spectrom. 2019 Aug;54(8):655-666. doi: 10.1002/jms.4379.
Soluble extractives in wood function to protect living trees from destructive agents and also contribute to wood color and fragrance. Some extractive components have biological activities with medical applications. They also play important roles in wood processing and related applications. To increase the knowledge of wood chemistry, maple and oak were extracted by water. Ultraviolet/visible (UV/vis) spectroscopy indicated the presence of a phenolic compound, resorcinol, in maple extractives having higher molecular mass and more aromatic components than oak extractives. Negative and positive electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR-MS) identified thousands of formulas in the two samples in the m/z range of 200 to 800. They mainly fall into the lignin-like, carbohydrate-like, and tannin-like compound categories. The top 25 peaks (ie, formulas) with the highest relative magnitude in negative ESI represented nearly 50% of the summed total spectral magnitude of all formulas assigned in the maple and oak extractives. Furthermore, the base peak (ie, most abundant peak) accounted for about 14% of the total abundance in each wood sample. Literature comparisons identified 17 of 20 formulas in the top five peaks of the four spectra as specific bioactive compounds in trees and other plants, implying the potential to explore utilization of maple and oak extractives for functional and medicinal applications. The various profiling of the top 25 peaks from the two samples also suggested the possible application of FT-ICR-MS for detecting chemical markers useful in profiling and identification of wood types and sources.
木材中的可溶性提取物具有保护活树免受破坏因子侵害的功能，还能赋予木材颜色和香气。一些提取物成分具有医学应用方面的生物活性。它们在木材加工及相关应用中也发挥着重要作用。为增加对木材化学的了解，用水对枫木和橡木进行了提取。紫外/可见（UV/vis）光谱表明，枫木提取物中存在一种酚类化合物间苯二酚，其分子量高于橡木提取物，且芳香成分更多。负离子和正离子电喷雾电离傅里叶变换离子回旋共振质谱（ESI FT-ICR-MS）在m/z范围为200至800的两个样品中鉴定出数千种分子式。它们主要分为类木质素、类碳水化合物和类单宁化合物类别。负离子电喷雾电离中相对强度最高的前25个峰（即分子式）占枫木和橡木提取物中所有已确定分子式总光谱强度的近50%。此外，基峰（即丰度最高的峰）在每个木材样品的总丰度中约占14%。文献比较表明，四个光谱中前五峰的20个分子式中有17个是树木和其他植物中的特定生物活性化合物，这意味着探索枫木和橡木提取物在功能和医药应用方面的利用潜力。对两个样品前25个峰的各种分析还表明，傅里叶变换离子回旋共振质谱可能用于检测有助于木材类型和来源分析及鉴定的化学标记物。
