Almurshidi Badria H, Van Court R C, Vega Gutierrez Sarath M, Harper Stacey, Harper Bryan, Robinson Seri C
Department of Wood Science, Oregon State University, Corvallis, OR 97333, USA.
Department of Toxicology, Oregon State University, Corvallis, OR 97331, USA.
J Fungi (Basel). 2021 Feb 22;7(2):155. doi: 10.3390/jof7020155.
Spalting fungal pigments have shown potential in technologies ranging from green energy generation to natural colorants. However, their unknown toxicity has been a barrier to industrial adoption. In order to gain an understanding of the safety of the pigments, zebrafish embryos were exposed to multiple forms of liquid media and solvent-extracted pigments with concentrations of purified pigment ranging from 0 to 50 mM from and Purified xylindein from . did not show toxicity at any tested concentration, while the red pigment dramada from was only associated with significant toxicity above 23.2 uM. However, liquid cultures and pigment extracted into dichloromethane (DCM) showed toxicity, suggesting the co-production of bioactive secondary metabolites. Future research on purification and the bioavailability of the red dramada pigment will be important to identify appropriate use; however, purified forms of the blue-green pigment xylindein are likely safe for use across industries. This opens the door to the adoption of green technologies based on these pigments, with potential to replace synthetic colorants and less stable natural pigments.
裂殖真菌色素在从绿色能源生产到天然色素等多种技术领域都展现出了潜力。然而，其未知的毒性一直是阻碍其在工业中应用的障碍。为了了解这些色素的安全性，斑马鱼胚胎被暴露于多种液体培养基和溶剂萃取的色素中，纯化色素的浓度范围为0至50 mM，分别来自[具体来源1]和[具体来源2]。从[具体来源3]纯化得到的木林菌素在任何测试浓度下均未显示出毒性，而来自[具体来源4]的红色色素dramada仅在浓度高于23.2 μM时才具有显著毒性。然而，液体培养物和萃取到二氯甲烷（DCM）中的色素显示出毒性，这表明生物活性次生代谢产物是共同产生的。未来对红色dramada色素的纯化和生物利用度的研究对于确定其合适用途至关重要；然而，蓝绿色色素木林菌素的纯化形式在各个行业中使用可能是安全的。这为基于这些色素的绿色技术的应用打开了大门，有可能取代合成色素和稳定性较差的天然色素。
