Obuchi Tomoko, Osada Masahiro, Ozawa Takeyuki, Nakagawa Hiroshi, Hayashi Michiko, Akiyama Kiyoshi, Sakagami Nobutada, Miura Ryotaro, Geshi Masaya, Ushijima Hitoshi
Department of Applied Life Science, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan.
Present: Division of Animal Reproduction, Maebashi Institute of Animal Science, Livestock Improvement Association of Japan (LIAJ), Gunma 371-0121, Japan.
J Reprod Dev. 2019 Aug 9;65(4):345-352. doi: 10.1262/jrd.2019-028. Epub 2019 Jun 10.
This study was conducted to evaluate and compare the economic benefits of different embryo sexing methods, based on the cost per female dairy calf produced. Female calves were produced from four kinds of female embryos: (1) those collected from superstimulated donors at 7-8 days after artificial insemination (AI) with X-sorted semen; (2) those sex-determined by loop-mediated isothermal amplification assay of a biopsy sample of embryos collected from superstimulated donors after AI with conventional unsorted semen; (3) those obtained by invitro embryo production (IVEP), using X-sorted semen and in vitro-matured oocytes collected from donors by ovum pick-up (OPU); and (4) those obtained by IVEP, using X-sorted semen and oocytes collected by OPU after dominant follicle ablation and follicle growth stimulation of the donors. The respective productivities of female calves per technical service and the total production cost per female calf of each sexing method were compared. The production cost per female calf (66,537 JPY), as calculated from the number of female calves per service (1.30), pregnancy rate of transfer (42.9%), rate of female calves obtained (92.9%), and total cost of the method (56,643 JPY plus embryo transfer fee), was less for IVEP with X-sorted semen and follicular growth-stimulated (FGS) oocytes than for the other groups (P < 0.05). The results demonstrate that embryo production with X-sorted semen and FGS oocytes provides a more efficient method for producing female calves than the other embryo sexing methods.
本研究旨在基于每头雌性奶牛犊牛的生产成本，评估和比较不同胚胎性别鉴定方法的经济效益。雌性犊牛由四种雌性胚胎产生：（1）在人工授精（AI）后7-8天，从经超排刺激的供体采集的、使用X分选精液的胚胎；（2）通过环介导等温扩增法对经AI后从经超排刺激的供体采集的胚胎活检样本进行性别鉴定的胚胎；（3）通过体外胚胎生产（IVEP）获得的胚胎，使用X分选精液和通过采卵（OPU）从供体采集的体外成熟卵母细胞；（4）通过IVEP获得的胚胎，使用X分选精液和在供体优势卵泡消融和卵泡生长刺激后通过OPU采集的卵母细胞。比较了每种性别鉴定方法每次技术服务的雌性犊牛生产率以及每头雌性犊牛的总生产成本。根据每次服务的雌性犊牛数量（1.30）、移植妊娠率（42.9%）、获得雌性犊牛的比率（92.9%）以及该方法的总成本（56,643日元加胚胎移植费用）计算得出，使用X分选精液和卵泡生长刺激（FGS）卵母细胞的IVEP方法每头雌性犊牛的生产成本（66,537日元）低于其他组（P<0.05）。结果表明，与其他胚胎性别鉴定方法相比，使用X分选精液和FGS卵母细胞进行胚胎生产是生产雌性犊牛更有效的方法。
