Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Port Blair 744103, Andaman and Nicobar Islands, India.
Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Port Blair 744103, Andaman and Nicobar Islands, India.
Mar Pollut Bull. 2019 Jul;144:105-116. doi: 10.1016/j.marpolbul.2019.05.011. Epub 2019 May 16.
Vibrio species are widely distributed in the estuarine and coastal waters that possess the greatest threat to human health worldwide. In this study it is aimed to isolate and observe the abundance of Vibrio sp. and prevalence of biomarker genes and antibiotic resistance profile of V. cholerae isolated from the Port Blair bays of South Andaman. A total of 56 water samples were collected from the seven sampling stations of Port Blair bays in which maximum number of Vibrio sp. population density (1.78 × 10) was recorded in Phoenix Bay. Among the 786 isolates 57.38% of the isolates were confirmed as Vibrio sp., Vibrio cholerae and Vibrio parahaemolyticus. PCR results revealed that the prevalence of biomarker genes was recorded maximum in the isolates from Phoenix Bay and Junglighat Bay samples. Upon further analysis, it was observed that the prevalence of hlyA gene (215 bp), was found to be the most widespread biomarker determinant in 84.17% of isolates. Major virulence determinants; ctxA, ompU and toxR genes were not detected in V. cholerae isolates from Port Blair bays. Maximum antibiotic resistance pattern was observed in Phoenix Bay isolates and maximum number of V. cholerae isolates was resistance to tetracycline (60.76%). Cluster and Principal Component Analysis were employed to understand the diversity and distribution of Vibrio isolates and its biomarker genes. Upon PCA analysis seasonal influence was not much perceived in Vibrio species diversity in Port Blair bays and the lack of significant difference in the detection of species diversity in this study is due to resemblance in geographical conditions and sources of pollution.
弧菌属广泛分布于具有全球最大人类健康威胁的港湾和沿海水域。本研究旨在从南安达曼的布莱尔港湾分离和观察弧菌属的丰度和生物标志物基因的流行率以及霍乱弧菌的抗生素耐药谱。从布莱尔港湾的七个采样站共采集了 56 个水样，其中凤凰湾的弧菌属种群密度最高（1.78×10）。在 786 个分离株中，57.38%的分离株被确认为弧菌属、霍乱弧菌和副溶血性弧菌。PCR 结果显示，凤凰湾和荣基拉哈特湾样本中的分离株的生物标志物基因流行率最高。进一步分析表明，hlyA 基因（215bp）的流行率在 84.17%的分离株中是最广泛的生物标志物决定因素。主要毒力决定因素 ctxA、ompU 和 toxR 基因未在布莱尔港湾的霍乱弧菌分离株中检测到。凤凰湾分离株表现出最大的抗生素耐药模式，最大数量的霍乱弧菌分离株对四环素（60.76%）耐药。聚类和主成分分析用于了解布莱尔港湾弧菌分离株及其生物标志物基因的多样性和分布。在 PCA 分析中，布莱尔港湾的弧菌物种多样性没有受到季节影响，而且本研究中物种多样性的检测没有显著差异，这是由于地理位置和污染来源相似所致。
