从乳制品和水样中分离的弧菌属的分子特征。
Molecular characterization of Vibrio species isolated from dairy and water samples.
机构信息
Department of Food Hygiene and Control (Milk Hygiene), Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Department of Cell and Tissues, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
出版信息
Sci Rep. 2023 Sep 16;13(1):15368. doi: 10.1038/s41598-023-42334-4.
Vibrio species can cause foodborne infections and lead to serious gastrointestinal illnesses. The purpose of this research was to detect the Vibrio cholerae and Vibrio parahaemolyticus in raw milk, dairy products, and water samples. Also, it investigated the virulence factors, antibiotic resistance and biofilm formation in isolated bacteria. Conventional and molecular approaches were used to identify the isolates in this study. Vibrio species were detected in 5% of the samples. Vibrio cholerae and Vibrio parahaemolyticus were isolated from 1.25 and 1.5%, respectively, of the total samples. Penicillin resistance was detected in all strains of Vibrio cholerae and Vibrio parahaemolyticus, with a MAR index ranging from 0.16 to 0.5. Four isolates were moderate biofilm producer and three of them were MDR. When Vibrio cholerae was screened for virulence genes, ctxAB, hlyA, and tcpA were found in 80, 60, and 80% of isolates, respectively. However, tdh + /trh + associated-virulence genes were found in 33.3% of Vibrio parahaemolyticus isolates.
弧菌属可引起食源性感染，并导致严重的胃肠道疾病。本研究旨在检测生奶、乳制品和水样中的霍乱弧菌和副溶血性弧菌。此外，还研究了分离菌的毒力因子、抗生素耐药性和生物膜形成。本研究采用常规和分子方法对分离株进行鉴定。在 5%的样本中检测到弧菌属。霍乱弧菌和副溶血性弧菌分别从总样本的 1.25%和 1.5%中分离出来。所有霍乱弧菌和副溶血性弧菌菌株均对青霉素耐药，MAR 指数在 0.16 至 0.5 之间。有 4 株为中度生物膜产生菌，其中 3 株为 MDR。当对霍乱弧菌进行毒力基因筛查时，发现 ctxAB、hlyA 和 tcpA 分别在 80%、60%和 80%的分离株中存在。然而，tdh+ / trh+相关的毒力基因在 33.3%的副溶血性弧菌分离株中存在。
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