乙二醇壳聚糖-荧光素异硫氰酸酯缀合物的荧光性质及其在生物成像材料中的应用。

Fluorescent property of glycol chitosan-fluorescein isothiocyanate conjugate for bio-imaging material.

机构信息

Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon 305-764, South Korea.

出版信息

Int J Biol Macromol. 2019 Aug 15;135:1217-1221. doi: 10.1016/j.ijbiomac.2019.06.038. Epub 2019 Jun 8.

DOI:10.1016/j.ijbiomac.2019.06.038

PMID:31181277

Abstract

Bio-imaging is an effective method for visualizing the biological responses and pharmacological responses in living organisms. In general, bio-imaging materials stain the cell membrane through electrostatic interaction and hydrogen bonding. However, the current commercial bio-imaging materials have several disadvantages, such as weak intensity, poor resolution, and high price. In this study, a novel bio-imaging material with high fluorescent intensity and water solubility was prepared using glycol chitosan. The chemical reaction between the amino group of glycol chitosan (GC) and the isothiocyanate group of fluorescein isothiocyanate (FITC) was monitored in order to synthesize glycol chitosan-fluorescein isothiocyanate (GC-FITC) conjugate. Then, the applicability of GC-FITC as a bio-imaging candidate was examined by comparing the fluorescence intensity of GC-FITC with that of commercial bio-imaging materials (dextran-FITC and BSA-FITC).

摘要

生物成像是一种有效的方法，可用于观察生物体内的生物反应和药理反应。一般来说，生物成像材料通过静电相互作用和氢键来染色细胞膜。然而，目前的商业生物成像材料存在一些缺点，如强度弱、分辨率差和价格高。在这项研究中，使用了乙二醇壳聚糖来制备一种具有高荧光强度和水溶性的新型生物成像材料。通过监测乙二醇壳聚糖（GC）的氨基与异硫氰酸荧光素（FITC）的异硫氰酸酯基团之间的化学反应，合成了乙二醇壳聚糖-异硫氰酸荧光素（GC-FITC）缀合物。然后，通过比较 GC-FITC 与商业生物成像材料（葡聚糖-FITC 和 BSA-FITC）的荧光强度，来检验 GC-FITC 作为生物成像候选物的适用性。