参与DNA代谢的枯草芽孢杆菌基因的分子克隆

Molecular cloning of Bacillus subtilis genes involved in DNA metabolism.

作者信息

Perego M, Ferrari E, Bassi M T, Galizzi A, Mazza P

机构信息

Dipartimento di Genetica e Microbiologia, A. Buzzati-Traverso, Università di Pavia, Italy.

出版信息

Mol Gen Genet. 1987 Aug;209(1):8-14. doi: 10.1007/BF00329829.

DOI:10.1007/BF00329829

PMID:3118144

Abstract

Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A lambda recombinant clone carrying a chromosomal fragment able to transform dnaC mutants was obtained from a lambda Charon 4A library. A restriction map of the cloned DNA fragments was constructed. The 11.3 kb cloned DNA fragment of plasmid pMP60-13 containing the wild-type sequence of dnaG5 was shown to transform a recF33 mutant as well.

摘要

从构建于质粒pJH101的文库中分离出不同的克隆，这些克隆携带能够将枯草芽孢杆菌突变体dnaA13、dnaB19、dnaG5、recG40和polA42转化为野生型表型的染色体DNA片段。从λ噬菌体Charon 4A文库中获得了一个携带能够转化dnaC突变体的染色体片段的λ重组克隆。构建了克隆DNA片段的限制性图谱。结果表明，含有dnaG5野生型序列的质粒pMP60 - 13的11.3 kb克隆DNA片段也能转化recF33突变体。