Ferrari F A, Nguyen A, Lang D, Hoch J A
J Bacteriol. 1983 Jun;154(3):1513-5. doi: 10.1128/jb.154.3.1513-1515.1983.
A plasmid useful for locating the chromosomal site of cloned DNA fragments that lack intrinsic genetic activity, for insertional mutagenesis, for single-copy complementation, and for dominance studies was constructed. Some plasmids containing Bacillus subtilis DNA were only active in transformation when the tetracycline resistance determinant of the plasmid was inactivated. The results suggest that production of the tetracycline gene product is lethal to B. subtilis.
构建了一种质粒，该质粒可用于定位缺乏内在遗传活性的克隆DNA片段的染色体位点、用于插入诱变、用于单拷贝互补以及用于显性研究。一些含有枯草芽孢杆菌DNA的质粒，只有在质粒的四环素抗性决定簇失活时才在转化中具有活性。结果表明，四环素基因产物的产生对枯草芽孢杆菌是致死的。
