参与芽孢萌发的枯草芽孢杆菌基因的分子克隆。

Molecular cloning of a Bacillus subtilis gene involved in spore outgrowth.

作者信息

Ferrari E, Scoffone F, Ciarrocchi G, Galizzi A

出版信息

J Gen Microbiol. 1985 Oct;131(10):2831-8. doi: 10.1099/00221287-131-10-2831.

Abstract

A lambda Charon 4A derivative carrying the outB gene of Bacillus subtilis has been identified by transformation of a B. subtilis mutant temperature-sensitive in spore outgrowth. The cloned region is a single EcoRI fragment 14 kb in length. In addition to outB, the cloned DNA includes at least part of the amyE and aroI loci.

摘要

通过对芽孢杆菌芽孢萌发温度敏感型突变体进行转化，已鉴定出携带枯草芽孢杆菌outB基因的λ噬菌体Charon 4A衍生物。克隆区域是一个长度为14 kb的单一EcoRI片段。除outB外，克隆的DNA至少包括amyE和aroI位点的一部分。