De Los Angeles Alejandro
Yale University School of Medicine, New Haven, Connecticut.
Curr Protoc Stem Cell Biol. 2019 Sep;50(1):e87. doi: 10.1002/cpsc.87. Epub 2019 Jun 11.
Pluripotency refers to the capacity of single cells to form derivatives of the three germ layers-ectoderm, mesoderm, and endoderm. Pluripotency can be captured in vitro as a spectrum of pluripotent stem cell states stabilized in specialized laboratory conditions. The recent discovery that pluripotent stem cells can colonize the embryos of distantly related animal organisms could, with further refinement, enable the generation of chimeric embryos composed of cells of human and animal origin. If achievable, the production of human-animal chimeras will open up new opportunities for regenerative medicine, facilitating human disease modeling and human organ generation inside large animals. However, the generation of human-animal interspecies chimeras is anticipated to require human chimera-competent pluripotent stem cells. Thus, it remains imperative to examine the pluripotency continuum more closely in light of advances that will facilitate the production of human-animal chimeras. This piece will review the current understanding of the pluripotency continuum and interspecies chimeras. © 2019 by John Wiley & Sons, Inc.
多能性是指单个细胞形成三个胚层（外胚层、中胚层和内胚层）衍生物的能力。多能性可以在体外被捕获，表现为在专门实验室条件下稳定的一系列多能干细胞状态。最近发现多能干细胞可以在远缘动物生物体的胚胎中定植，随着进一步完善，这可能会使由人类和动物来源的细胞组成的嵌合胚胎得以产生。如果能够实现，人类-动物嵌合体的产生将为再生医学带来新的机遇，促进人类疾病建模以及在大型动物体内生成人类器官。然而，人类-动物种间嵌合体的产生预计需要具备嵌合能力的人类多能干细胞。因此，鉴于有助于人类-动物嵌合体产生的进展，更深入地研究多能性连续体仍然至关重要。本文将综述目前对多能性连续体和种间嵌合体的理解。© 2019约翰威立父子公司版权所有
