Division of Urology, Department of Surgery, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.
Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.
J Med Microbiol. 2019 Aug;68(8):1244-1252. doi: 10.1099/jmm.0.001017. Epub 2019 Jun 11.
The high incidence of urinary tract infection (UTI) among women and children, in combination with a lack of antibiotic efficacy with regard to pathogen eradication and recurrence prevention, as well as the negative side effects associated with antibiotics, has led researchers to explore the role of non-steroidal anti-inflammatory drugs as a primary management strategy. The aim of this study was to determine whether ibuprofen (IBU) or one of its major metabolites, 2-carboxyibuprofen (CIBU), could affect the growth and adhesion of the two most common uropathogens, Escherichia coli and Enterococcus faecalis. The bacterial growth and adhesion to the urothelial cells of E. coli UTI89 and E. faecalis 1131 in the presence of physiologically relevant concentrations of IBU and CIBU were assessed. The effect of IBU on bacterial adhesion to urothelial cells was also assessed following exposure to trimethoprim/sulfamethoxazole (TMP/SMX) and nitrofurantoin. Bacterial growth was not affected by IBU. Further, only at high levels of IBU not regularly found in the bladder was there a significant increase in E. faecalis 1131 attachment at growth inhibitory concentrations of TMP/SMX. There was no effect on the attachment of E. faecalis or E. coli to urothelial cells in the presence of nitrofurantoin. These studies indicate that the beneficial effects of IBU for UTI management are likely mediated through its anti-inflammatory properties rather than direct interactions with uropathogens in the bladder.
女性和儿童尿路感染（UTI）的高发率，加上抗生素在消除病原体和预防复发方面的疗效不足，以及抗生素的负面副作用，促使研究人员探索非甾体抗炎药作为主要管理策略的作用。本研究旨在确定布洛芬（IBU）或其主要代谢物 2-羧基布洛芬（CIBU）是否会影响两种最常见的尿路病原体大肠杆菌和粪肠球菌的生长和黏附。在生理相关浓度的 IBU 和 CIBU 存在下，评估了 IBU 和 CIBU 对 E. coli UTI89 和 E. faecalis 1131 细菌生长和黏附到尿路上皮细胞的影响。还评估了 IBU 对暴露于甲氧苄啶/磺胺甲噁唑（TMP/SMX）和呋喃妥因后细菌黏附到尿路上皮细胞的影响。IBU 对细菌生长没有影响。此外，只有在膀胱中未发现的高水平 IBU 才会在 TMP/SMX 的生长抑制浓度下显著增加粪肠球菌 1131 的黏附。在呋喃妥因存在的情况下，对粪肠球菌或大肠杆菌与尿路上皮细胞的黏附没有影响。这些研究表明，IBU 对 UTI 管理的有益影响可能是通过其抗炎特性介导的，而不是与膀胱中的尿路病原体直接相互作用。
