Scotland Kymora B, Kung Sonia Hy, Chew Ben H, Lange Dirk
Department of Urology, University of California Los Angeles, Los Angeles, CA 90024, USA.
Vancouver Prostate Centre, Department of Urologic Sciences, University of British Columbia, Vancouver, BC V6H 3Z6, Canada.
Pathogens. 2020 Sep 18;9(9):764. doi: 10.3390/pathogens9090764.
Despite routine implementation in urology, indwelling ureteral stents pose as a nidus for infection. Conditioning film accumulates on stents, which prime pathogen adhesion, promoting infectious biofilm formation. However, the extent to which conditioning film components play a role in facilitating bacterial adhesion and biofilm formation remains largely unknown. Here, we examined the interaction of previously identified stent-bound conditioning film components (fibrinogen, uromodulin, and albumin) with bacterial uropathogens. Cytoscopically removed stents were incubated with common uropathogens (, , and ). Immunofluorescent double staining was performed to study the localization of uropathogens relative to stent-bound conditioning film proteins. Conditioning film components were identified on the external stent surface with some deposition in the inner lumen. Bacteria co-localized with fibrinogen, uromodulin, and albumin, suggesting a potential mechanism for stent-associated infections. Here, we determine strong co-localization between common uropathogenic bacterial species with prominent conditioning film components on ureteral stents. Further functional validation of interactions amongst these uropathogens and conditioning film proteins may enhance clinical management for stent-associated infections and development of improved stent technologies.
尽管输尿管留置支架在泌尿外科已常规使用，但它却是感染的病灶。调理膜在支架上积聚，引发病原体黏附，促进感染性生物膜形成。然而，调理膜成分在促进细菌黏附和生物膜形成中所起的作用程度仍 largely 未知。在此，我们研究了先前鉴定出的与支架结合的调理膜成分（纤维蛋白原、尿调节蛋白和白蛋白）与尿路致病菌之间的相互作用。通过膀胱镜取出的支架与常见尿路致病菌（、、和）一起孵育。进行免疫荧光双重染色以研究尿路致病菌相对于与支架结合的调理膜蛋白的定位。在支架外表面鉴定出调理膜成分，在内腔有一些沉积。细菌与纤维蛋白原、尿调节蛋白和白蛋白共定位，提示了支架相关感染的潜在机制。在此，我们确定了常见尿路致病菌种与输尿管支架上突出的调理膜成分之间有强烈的共定位。这些尿路致病菌与调理膜蛋白之间相互作用的进一步功能验证可能会加强对支架相关感染的临床管理并推动改进支架技术的发展。
需注意，原文中存在部分未明确的内容（如“、、和”），翻译时保留了原文形式。
