School of Medicine, South China University of Technology, Guangzhou 510006, China and State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou 510640, China.
State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou 510640, China.
Chem Commun (Camb). 2019 Jul 4;55(52):7458-7461. doi: 10.1039/c9cc02782c. Epub 2019 Jun 11.
The development of a sensitive and reliable method for the detection of bioaccumulated heavy metal toxins is highly desirable for biotoxicity evaluation. However, the conventional biotoxicity evaluation method based on luminescent bacteria suffers from only being able to detect the overall toxicity without selectivity in light-off detection mode. Although various synthetic fluorescent probes have been developed for the selective detection of heavy metal ions, they usually suffer from aggregation-caused quenching after local accumulation in biological systems. To tackle these challenges, we herein develop a dual detection strategy for bioaccumulated Hg based on turn-off of the bioluminescence of P. phosphoreum bacteria by disrupting the quorum sensing system and turn-on of the photoluminescence of an aggregation-induced emission (AIE) probe by forming aggregates with Hg inside the bacteria. It is expected that the dual detection strategy would find broad applications in the evaluation of bioaccumulated toxins.
开发一种灵敏可靠的方法来检测生物累积的重金属毒素对于生物毒性评估是非常需要的。然而，基于发光细菌的传统生物毒性评估方法只能在关闭检测模式下检测整体毒性，而没有选择性。尽管已经开发了各种合成荧光探针用于选择性检测重金属离子，但它们通常在生物系统中局部积累后会因聚集引起的猝灭而失效。为了解决这些挑战，我们在此开发了一种基于双检测策略的生物累积汞，该策略基于破坏 P. phosphoreum 细菌的群体感应系统来关闭生物发光，以及通过在细菌内与汞形成聚集体来开启聚集诱导发射（AIE）探针的光致发光。预计这种双重检测策略将在生物累积毒素的评估中得到广泛应用。
