Cutler J, Williamson S M, Rae R
Liverpool John Moores University, School of Natural Sciences and Psychology, Byrom Street, Liverpool, L33AF, United Kingdom.
Liverpool John Moores University, School of Natural Sciences and Psychology, Byrom Street, Liverpool, L33AF, United Kingdom.
Behav Processes. 2019 Aug;165:1-3. doi: 10.1016/j.beproc.2019.06.009. Epub 2019 Jun 8.
The nematode Phasmarhabditis hermaphrodita can infect and kill many species of slugs and has been formulated into a biological control agent for farmers and gardeners. P. hermaphrodita can manipulate the behaviour of slugs, making those infected move to areas where the nematode is present. Research suggests P. hermaphrodita uses manipulation of biogenic amines to achieve this, however the exact role of serotonin and dopamine needs further elucidation. Here we fed slugs Deroceras invadens (uninfected and infected with P. hermaphrodita) apomorphine, sertraline and haloperidol and observed their behaviour when given a choice between a P. hermaphrodita infested habitat, or a parasite free area of soil. In contrast to their usual P. hermaphrodita avoidance behaviour, uninfected D. invadens fed sertraline were attracted to the nematodes and conversely those fed haloperidol avoided the nematodes. D. invadens fed apomorphine were recorded equally on the control and nematode side. D. invadens pre-infected with P. hermaphrodita fed sertraline and apomorphine were found significantly more on the side with the nematodes. However, suppressing dopaminergic signalling through feeding with haloperidol abrogated this attraction and slugs were found on both sides. These results demonstrate that serotonin and dopamine are potential regulators of behavioural manipulation by P. hermaphrodita.
线虫雌雄同体拟阿勇蛲虫可感染并杀死多种蛞蝓，现已被配制成一种生物防治剂供农民和园丁使用。雌雄同体拟阿勇蛲虫能够操控蛞蝓的行为，使受感染的蛞蝓移动到该线虫存在的区域。研究表明，雌雄同体拟阿勇蛲虫利用对生物胺的操控来实现这一点，然而血清素和多巴胺的确切作用仍需进一步阐明。在此，我们给入侵蛞蝓（未感染和感染了雌雄同体拟阿勇蛲虫）喂食阿扑吗啡、舍曲林和氟哌啶醇，并观察它们在有雌雄同体拟阿勇蛲虫侵染的栖息地或无寄生虫的土壤区域之间做出选择时的行为。与它们通常对雌雄同体拟阿勇蛲虫的回避行为相反，喂食舍曲林的未感染入侵蛞蝓被线虫吸引，相反，喂食氟哌啶醇的则回避线虫。喂食阿扑吗啡的入侵蛞蝓在对照侧和线虫侧的记录数量相同。预先感染了雌雄同体拟阿勇蛲虫并喂食舍曲林和阿扑吗啡的入侵蛞蝓，在线虫所在一侧被发现的数量显著更多。然而，通过喂食氟哌啶醇抑制多巴胺能信号传导消除了这种吸引力，蛞蝓在两侧都被发现。这些结果表明，血清素和多巴胺是雌雄同体拟阿勇蛲虫行为操控的潜在调节因子。
