Liao Shu, Yu Chaxiu, Liu Hucheng, Zhang Congkai, Li Yong, Zhong Xiaojun
Department of Medical Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China,
Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.
Onco Targets Ther. 2019 May 24;12:4099-4107. doi: 10.2147/OTT.S185156. eCollection 2019.
This study aimed to explore the effect of long non-coding RNA (LncRNA) H19 on the proliferation and invasion of lung carcinoma cells A549, and to determine its molecular targets.
A549 cells were with either LncRNA H19 or LncRNA H19 shRNA, and the expression levels of LncRNA H19 were evaluated by quantitative real-time PCR (RT-PCR). We measured cell proliferation using the CCK-8 assay, cell counting assays, and colony formation assay in response to shLncRNA H19-2. Cell migration and invasion were assessed by wound healing assay and Transwell assay, respectively. The mRNA and protein expression levels of E-cadherin, N-cadherin, and vimentin were determined by RT-PCR and western blot, respectively.
The three LncRNA H19 shRNAs used in our study significantly reduced the expression levels of LncRNA H19 in A549 cells (<0.05). Moreover, LncRNA H19 shRNA 2 (shLncRNA-2) was the most potent inhibitor of LncRNA H19 expression, and was selected for further experimentation. Transfection with shLncRNA H19-2 significantly decreased the proliferation, migration, and invasion of A549 cells, while overexpression of LncRNA H19 had the opposite effect in these cells (<0.05). In response to shLncRNA H19-2, the expression levels of E-cadherin were notably elevated (<0.05), while the expression levels of N-cadherin and vimentin were decreased (<0.05). In contrast, overexpression of LncRNA H19 induced the expression of E-cadherin, and blocked the expression of N-cadherin, and vimentin (<0.05).
Our results suggest that LncRNA H19 mediates the proliferation and invasion of lung cancer cells via upregulation of N-cadherin and vimentin, and downregulation of E-cadherin.
本研究旨在探讨长链非编码RNA（LncRNA）H19对肺癌细胞A549增殖和侵袭的影响，并确定其分子靶点。
将A549细胞分别转染LncRNA H19或LncRNA H19短发夹RNA（shRNA），采用定量实时聚合酶链反应（RT-PCR）评估LncRNA H19的表达水平。我们使用CCK-8法、细胞计数法和集落形成试验来检测shLncRNA H19-2对细胞增殖的影响。分别通过伤口愈合试验和Transwell试验评估细胞迁移和侵袭能力。通过RT-PCR和蛋白质印迹法分别测定E-钙黏蛋白、N-钙黏蛋白和波形蛋白的mRNA和蛋白质表达水平。
我们研究中使用的三种LncRNA H19 shRNA均显著降低了A549细胞中LncRNA H19的表达水平（<0.05）。此外，LncRNA H19 shRNA 2（shLncRNA-2）是LncRNA H19表达的最有效抑制剂，并被选用于进一步实验。转染shLncRNA H19-2显著降低了A549细胞的增殖、迁移和侵袭能力，而LncRNA H19的过表达在这些细胞中则产生相反的效果（<0.叭）。在shLncRNA H19-2作用下，E-钙黏蛋白的表达水平显著升高（<0.05），而N-钙黏蛋白和波形蛋白的表达水平降低（<0.05）。相反，LncRNA H19的过表达诱导了E-钙黏蛋白的表达，并阻断了N-钙黏蛋白和波形蛋白的表达（<0.05）。
我们的结果表明，LncRNA H19通过上调N-钙黏蛋白和波形蛋白以及下调E-钙黏蛋白来介导肺癌细胞的增殖和侵袭。
