Hunt J M, Guo C J, Desai P A
Department of Pathology and Laboratory Medicine, University of Texas Medical School, Houston 77225.
Cancer Lett. 1987 Nov;37(3):285-91. doi: 10.1016/0304-3835(87)90113-3.
Rat epoxide hydrolase (EH) (EC 3.3.2.3) is elevated in cells of premalignant liver lesions, and variable EH activity has been reported for hepatocellular carcinomas. To facilitate detection of altered EH levels in liver cells, an immunoblotting method was devised. Rabbit antiserum specific for rat EH was prepared and used to detect EH extracted from suspensions of normal liver cells and from hepatoma cell lines. Compared with normal liver cells, 3 rat hepatoma cell lines, 7777, HTC and 17X, showed virtually undetectable EH levels by immunoblotting. The immunoblotting results rule out the possibility that very low EH enzymatic activity in the hepatoma cells results from production of normal amounts of non-functional enzyme protein.
大鼠环氧化物水解酶（EH）（EC 3.3.2.3）在前恶性肝损伤细胞中水平升高，并且已有报道称肝细胞癌的EH活性存在差异。为便于检测肝细胞中EH水平的变化，设计了一种免疫印迹法。制备了对大鼠EH具有特异性的兔抗血清，并用于检测从正常肝细胞悬液和肝癌细胞系中提取的EH。与正常肝细胞相比，3种大鼠肝癌细胞系7777、HTC和17X通过免疫印迹显示几乎检测不到EH水平。免疫印迹结果排除了肝癌细胞中极低的EH酶活性是由正常量的无功能酶蛋白产生所致的可能性。
