Wolf C R, Oesch F, Timms C, Guenthner T, Hartmann R, Maruhn M, Burger R
FEBS Lett. 1983 Jul 4;157(2):271-6. doi: 10.1016/0014-5793(83)80560-2.
Monoclonal antibodies have been prepared against rat liver epoxide hydrolase (EH), some of which gave precipitation lines on immunodiffusion against pure EH suggesting the presence of repetitive structural domains on the enzyme. Using ELISA, with polyclonal antibodies to rat and rabbit liver EH, reactivity and therefore structural similarities between EH of all species tested, including human, were observed. This was in contrast to immunodiffusion results demonstrating the limitations of the latter technique. Using monoclonal antibodies in ELISA, greatest structural similarity was between rat, mouse, and Syrian hamster EH and relatively little between rat and human. Two of the antibodies reacted with nearly all species tested and may be directed towards critical sites on the enzyme. This and most of the EH molecule would appear to be localised on the cytoplasmic surface of the endoplasmic reticulum.
已经制备了针对大鼠肝脏环氧化物水解酶（EH）的单克隆抗体，其中一些在与纯EH的免疫扩散实验中产生了沉淀线，这表明该酶上存在重复的结构域。使用酶联免疫吸附测定（ELISA），用针对大鼠和兔肝脏EH的多克隆抗体，观察到包括人类在内的所有测试物种的EH之间的反应性以及因此的结构相似性。这与免疫扩散结果形成对比，后者证明了该技术的局限性。在ELISA中使用单克隆抗体，大鼠、小鼠和叙利亚仓鼠的EH之间结构相似性最大，而大鼠和人类之间的相似性相对较小。其中两种抗体与几乎所有测试物种都有反应，可能针对该酶上的关键位点。并且大部分EH分子似乎定位于内质网的细胞质表面。
