Twardowski J, Wójczyk B, Nowak I
Acta Physiol Hung. 1987;69(1):85-94.
The different forms of acid phosphatase (EC 3.1.3.2) in rat liver homogenates, lysosomal, mitochondrial, microsomal fractions and cytosol were studied with isoelectric focusing. Evidence is presented that isoelectric focusing of acid phosphatase in subcellular fractions shows individual changes and time related patterns. Mild autolysis shifted all enzyme activity peaks of isoelectric focusing patterns to the one at pH 7.04.
采用等电聚焦法研究了大鼠肝脏匀浆、溶酶体、线粒体、微粒体组分及胞质溶胶中不同形式的酸性磷酸酶(EC 3.1.3.2)。有证据表明,亚细胞组分中酸性磷酸酶的等电聚焦呈现出个体差异和与时间相关的模式。轻度自溶使等电聚焦图谱的所有酶活性峰都移向了pH 7.04处的峰。