Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas , Universidad Complutense de Madrid , E-28040 Madrid , Spain.
Centro de Investigaciones Biológicas, CSIC , E-28040 Madrid , Spain.
J Proteome Res. 2019 Aug 2;18(8):3052-3066. doi: 10.1021/acs.jproteome.9b00167. Epub 2019 Jun 27.
Olive pollen is a major allergenic source worldwide due to its extensive cultivation. We have combined available genomics data with a comprehensive proteomics approach to get the annotated olive tree ( L.) pollen proteome and define its complex allergenome. A total of 1907 proteins were identified by LC-MS/MS using predicted protein sequences from its genome. Most proteins (60%) were predicted to possess catalytic activity and be involved in metabolic processes. In total, 203 proteins belonging to 47 allergen families were found in olive pollen. A peptidyl-prolyl isomerase, cyclophilin, produced in , was found as a new olive pollen allergen (Ole e 15). Most Ole e 15-sensitized patients were children (63%) and showed strong IgE recognition to the allergen. Ole e 15 shared high sequence identity with other plant, animal, and fungal cyclophilins and presented high IgE cross-reactivity with pollen, plant food, and animal extracts.
由于广泛种植，橄榄花粉是世界范围内主要的过敏原来源。我们将可用的基因组学数据与全面的蛋白质组学方法相结合，得到了注释的橄榄树（L.）花粉蛋白质组，并定义了其复杂的过敏原组。通过 LC-MS/MS 使用其基因组的预测蛋白序列共鉴定了 1907 种蛋白质。大多数蛋白质（60%）被预测具有催化活性并参与代谢过程。在橄榄花粉中共发现 203 种属于 47 种过敏原家族的蛋白质。一种肽基脯氨酰 异构酶，环孢素，在 中产生，被发现为一种新的橄榄花粉过敏原（Ole e 15）。大多数对 Ole e 15 敏感的患者为儿童（63%），对过敏原表现出强烈的 IgE 识别。Ole e 15 与其他植物、动物和真菌的环孢素具有高度的序列同一性，并与花粉、植物性食物和动物性提取物表现出高的 IgE 交叉反应性。
