Guo Wei, Zhan Xiaodong, Jiang Feng, Xi Yilong
School of Ecology and Environment, Anhui Normal University, Wuhu, 241002, China.
Department of Parasitology, School of Basic Medicine, Wannan Medical College, Wuhu, 241002, China.
Proteome Sci. 2021 Sep 3;19(1):10. doi: 10.1186/s12953-021-00178-8.
Allergies caused by pollen from Populus deltoides are common, but the allergic components are still unclear.
The total proteins in pollen of P. deltoides were analyzed by proteomics, and the potential allergens were identified via the WHO/IUIS database and the allergenOnline database retrieval. One target protein was screened by bioinformatics and expressed in Escherichia coli. The biological activity of the expressed product was verified by animal experiments.
The total of 3929 proteins in pollen of P. deltoides were identified, and 46 potential allergens belonging to 10 protein families were recognized by database retrieval. B9N9W6 protein of Hsp70 family was screened by bioinformatics analysis and expressed successfully. ELISA showed that B9N9W6 can stimulate the immune system to produce specific IgE and promote the generation of IL-4. Flow cytometry showed that B9N9W6 can significantly stimulate the proliferation of CD4 T cells and promote the polarization of Th2 cells. The pathological sections of mice lung tissues indicated that alveolar destruction was more severe in the B9N9W6 group than that of extract group, and there were more inflammatory cells infiltration, mucus exudation and bleeding.
B9N9W6 is an important antigenic substance in the pollen of P. deltoides. Due to the conserved structure of Hsp70 family, more attention should be paid to the possibility of sensitization when Hsp70 from any pathogenic species is administered.
由三角叶杨花粉引起的过敏很常见，但过敏成分仍不清楚。
采用蛋白质组学方法分析三角叶杨花粉中的总蛋白，并通过WHO/IUIS数据库和AllergenOnline数据库检索鉴定潜在过敏原。通过生物信息学筛选出一种靶蛋白并在大肠杆菌中表达。通过动物实验验证表达产物的生物学活性。
鉴定出三角叶杨花粉中的3929种蛋白质，通过数据库检索识别出属于10个蛋白家族的46种潜在过敏原。通过生物信息学分析筛选出Hsp70家族的B9N9W6蛋白并成功表达。ELISA结果显示，B9N9W6可刺激免疫系统产生特异性IgE并促进IL-4的产生。流式细胞术结果显示，B9N9W6可显著刺激CD4 T细胞增殖并促进Th2细胞极化。小鼠肺组织病理切片显示，B9N9W6组肺泡破坏比提取物组更严重，炎症细胞浸润、黏液渗出和出血更多。
B9N9W6是三角叶杨花粉中的一种重要抗原物质。由于Hsp70家族结构保守，当使用来自任何致病物种的Hsp70时，应更加关注致敏的可能性。
