Ali Sanaa A, Ibrahim Nabaweya A, Mohammed Magdy M D, El-Hawary Seham, Refaat Esraa A
Therapeutic Chemistry Department, National Research Center, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.
Pharmacognosy Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt.
Heliyon. 2019 May 24;5(5):e01769. doi: 10.1016/j.heliyon.2019.e01769. eCollection 2019 May.
The present study discusses the isolation of ursolic acid from the chloroform extract of (Thunb) Steud fruits and its cytotoxic effect has been assessed was performed in different cells lines (A-549, MCF-7, HepG2) and using N-diethylnitrosamine. The obtained results revealed that ursolic acid showed significant cytotoxic activity on MCF-7 and HepG2 cell lines in comparison to Doxorubicin as a reference drug. Moreover, we have assessed the inhibitory effects of fruit chloroform extract and the isolated ursolic acid on hepatocarcinogenesis was carried out for the first time using N-diethylnitrosamine, where the group treated with ursolic acid given orally after 8 weeks of cancer induction showed the most significant results in comparison to the chloroform extract. The effect of ursolic acid on intoxicated rats caused significant restoration of most of the normal hepatocytes architecture with regular dark nuclei and the group treated with fruits showed remarkable results with improvement in biochemical analysis.
本研究讨论了从（Thunb）Steud果实的氯仿提取物中分离熊果酸，并评估了其细胞毒性作用。该实验在不同细胞系（A-549、MCF-7、HepG2）中进行，并使用N-二乙基亚硝胺。所得结果表明，与作为参考药物的阿霉素相比，熊果酸对MCF-7和HepG2细胞系显示出显著的细胞毒性活性。此外，我们首次使用N-二乙基亚硝胺评估了果实氯仿提取物和分离出的熊果酸对肝癌发生的抑制作用，其中在癌症诱导8周后口服熊果酸治疗的组与氯仿提取物相比显示出最显著的结果。熊果酸对中毒大鼠的作用使大多数正常肝细胞结构显著恢复，细胞核规则且深染，用果实治疗的组在生化分析方面有显著改善，结果显著。
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