McWhirt Joshua, Sathyanesan Monica, Sampath Dayalan, Newton Samuel S
Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, SD, 57069, USA.
Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, Sioux Falls VA Healthcare System, Sioux Falls, SD, 57105, USA.
Neurobiol Stress. 2019 Apr 30;10:100169. doi: 10.1016/j.ynstr.2019.100169. eCollection 2019 Feb.
The two strains of inbred mice, BALB/c and C57BL/6, are widely used in pre-clinical psychiatry research due to their differences in stress susceptibility. Gene profiling studies in these strains have implicated the inflammation pathway as the main contributor to these differences. We focused our attention on female mice and tested their response to 5- or 10-day exposure to restraint stress. We examined the stress induced changes in the regulation of 11 inflammatory cytokine genes and 12 glutamate receptor genes in the hippocampus of female BALB/c and C57BL/6 mice using quantitative PCR. Elevated proinflammatory cytokine genes include Tumor Necrosis Factor alpha (TNFa), nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB), Interleukin 1 alpha (IL1a), Interleukin 1 receptor (IL1R), Interleukin 10 receptor alpha subunit (IL10Ra), Interleukin 10 receptor beta subunit (IL10Rb), and tumor necrosis factor (TNF) super family members. Our results show that BALB/c and C57BL/6 mice differ in the genes induced in response to stress exposure and the level of gene regulation change. Our results show that the gene regulation in female BALB/c and C57BL/6 mice differs between strains in the genes regulated and the magnitude of the changes.
近交系小鼠BALB/c和C57BL/6这两个品系，由于其对应激易感性的差异，在临床前精神病学研究中被广泛使用。对这些品系的基因谱研究表明，炎症途径是造成这些差异的主要因素。我们将注意力集中在雌性小鼠身上，并测试了它们对5天或10天束缚应激暴露的反应。我们使用定量PCR检测了雌性BALB/c和C57BL/6小鼠海马体中11种炎性细胞因子基因和12种谷氨酸受体基因在应激诱导下的调控变化。促炎细胞因子基因升高的包括肿瘤坏死因子α（TNFα）、活化B细胞核因子κ轻链增强子（NFKB）、白细胞介素1α（IL1α）、白细胞介素1受体（IL1R）、白细胞介素10受体α亚基（IL10Ra）、白细胞介素10受体β亚基（IL10Rb）以及肿瘤坏死因子（TNF）超家族成员。我们的结果表明，BALB/c和C57BL/6小鼠在应激暴露诱导的基因以及基因调控变化水平上存在差异。我们的结果表明，雌性BALB/c和C57BL/6小鼠在基因调控方面存在品系差异，包括所调控基因以及变化幅度。
