Ohta T, Takasuka T, Ishibashi S, Ide T
Cell Struct Funct. 1985 Mar;10(1):37-46. doi: 10.1247/csf.10.37.
When the growth of serum-arrested GC-7 cells, a clone from African green monkey kidney, was induced by the addition of 10% calf serum, they began to enter S phase after 15-16 h. When stimulated cells were cultured in the presence of 0.6 micrograms/ml of cytochalasin D, the entrance into S phase was inhibited. Treatment of cells with cytochalasin D during the period earlier than 8 h or later than 11 h after the serum stimulation showed no or little inhibitory effect on the entrance of cells into S phase. Inhibition of the entrance into S phase was observed only when stimulated cells were treated with cytochalasin D during the periods including 9-10 h after stimulation. A rapid increase in protein synthesis occurred 9-12 h after the serum stimulation and was inhibited in the presence of cytochalasin D. These and other results suggested that in the course of the prereplicative process from Go through S phase only the stage around 9-10 h after the start of the cell cycle was sensitive to cytochalasin D and that the block of the cycle was correlated with the inhibition of protein synthesis at this stage.
当添加10%小牛血清诱导处于血清停滞状态的GC - 7细胞（一种来自非洲绿猴肾的克隆细胞）生长时，它们在15 - 16小时后开始进入S期。当在含有0.6微克/毫升细胞松弛素D的情况下培养受刺激的细胞时，进入S期受到抑制。在血清刺激后早于8小时或晚于11小时的时间段用细胞松弛素D处理细胞，对细胞进入S期没有或几乎没有抑制作用。仅当在刺激后9 - 10小时的时间段用细胞松弛素D处理受刺激的细胞时，才观察到进入S期受到抑制。血清刺激后9 - 12小时蛋白质合成迅速增加，并且在存在细胞松弛素D的情况下受到抑制。这些以及其他结果表明，在从G0期到S期的复制前过程中，仅细胞周期开始后约9 - 10小时的阶段对细胞松弛素D敏感，并且该阶段的周期阻滞与蛋白质合成的抑制相关。
