Matthews K R, Roberson J, Gillespie B E, Luther D A, Oliver S P
Department of Food Science, Rutgers University, New Brunswick, New Jersey 08903.
Institute of Agriculture, The University of Tennessee, Knoxville, Tennessee 37901, USA.
J Food Prot. 1997 Jun;60(6):686-688. doi: 10.4315/0362-028X-60.6.686.
Staphylococcus aureus is an etiological agent of a wide variety of human and animal infections. The majority of S. aureus are coagulase-positive; however, some may be atypical in that they do not produce coagulase. Incorrect identification of an isolate can impact implementation of effective treatment and/or control measures. In this study, polymerase chain reaction-based DNA fingerprinting was used to differentiate coagulase-positive Staphylococcus aureus (CPSA) from coagulase-negative Staphylococcus aureus (CNSA). A total of 29 CNSA and 50 CPSA were evaluated. PCR-based DNA fingerprinting differentiated CNSA from CPSA on the basis of visible observation and densitometric evaluation. The method is rapid and accurate, eliminating variability associated with conventional techniques.
金黄色葡萄球菌是多种人类和动物感染的病原体。大多数金黄色葡萄球菌是凝固酶阳性的；然而，有些可能是非典型的，因为它们不产生凝固酶。分离株的错误鉴定会影响有效治疗和/或控制措施的实施。在本研究中，基于聚合酶链反应的DNA指纹图谱被用于区分凝固酶阳性金黄色葡萄球菌（CPSA）和凝固酶阴性金黄色葡萄球菌（CNSA）。共评估了29株CNSA和50株CPSA。基于PCR的DNA指纹图谱通过肉眼观察和光密度评估来区分CNSA和CPSA。该方法快速准确，消除了与传统技术相关的变异性。
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