Karmakar Amit, Dua Parimal, Ghosh Chandradipa
Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Paschim Medinipur, West Bengal 721102, India.
Can J Infect Dis Med Microbiol. 2016;2016:9041636. doi: 10.1155/2016/9041636. Epub 2016 Apr 24.
Staphylococcus aureus is opportunistic human as well as animal pathogen that causes a variety of diseases. A total of 100 Staphylococcus aureus isolates were obtained from clinical samples derived from hospitalized patients. The presumptive Staphylococcus aureus clinical isolates were identified phenotypically by different biochemical tests. Molecular identification was done by PCR using species specific 16S rRNA primer pairs and finally 100 isolates were found to be positive as Staphylococcus aureus. Screened isolates were further analyzed by several microbiological diagnostics tests including gelatin hydrolysis, protease, and lipase tests. It was found that 78%, 81%, and 51% isolates were positive for gelatin hydrolysis, protease, and lipase activities, respectively. Antibiogram analysis of isolated Staphylococcus aureus strains with respect to different antimicrobial agents revealed resistance pattern ranging from 57 to 96%. Our study also shows 70% strains to be MRSA, 54.3% as VRSA, and 54.3% as both MRSA and VRSA. All the identified isolates were subjected to detection of mecA, nuc, and hlb genes and 70%, 84%, and 40% were found to harbour mecA, nuc, and hlb genes, respectively. The current investigation is highly important and informative for the high level multidrug resistant Staphylococcus aureus infections inclusive also of methicillin and vancomycin.
金黄色葡萄球菌是一种机会性人类和动物病原体，可引发多种疾病。从住院患者的临床样本中总共获得了100株金黄色葡萄球菌分离株。通过不同的生化试验对推定的金黄色葡萄球菌临床分离株进行表型鉴定。使用物种特异性16S rRNA引物对通过PCR进行分子鉴定，最终发现100株分离株为金黄色葡萄球菌阳性。对筛选出的分离株通过包括明胶水解试验、蛋白酶试验和脂肪酶试验在内的多种微生物诊断试验进行进一步分析。结果发现，分别有78%、81%和51%的分离株具有明胶水解活性、蛋白酶活性和脂肪酶活性。对分离出的金黄色葡萄球菌菌株针对不同抗菌剂的抗菌谱分析显示耐药率在57%至96%之间。我们的研究还表明，70%的菌株为耐甲氧西林金黄色葡萄球菌（MRSA），54.3%为耐万古霉素金黄色葡萄球菌（VRSA），54.3%同时为MRSA和VRSA。对所有鉴定出的分离株进行mecA、nuc和hlb基因检测，结果发现分别有70%、84%和40%的分离株携带mecA、nuc和hlb基因。当前的调查对于包括耐甲氧西林和耐万古霉素在内的高水平多重耐药金黄色葡萄球菌感染具有高度重要性和参考价值。
