Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, Málaga 29071, Spain.
Bioinformatics. 2019 Nov 1;35(22):4849-4850. doi: 10.1093/bioinformatics/btz462.
The oxidation of protein-bound methionine to form methionine sulfoxide has traditionally been regarded as an oxidative damage. However, growing evidences support the view of this reversible reaction also as a regulatory post-translational modification. Thus, the oxidation of methionine residues has been reported to have multiple and varied implications for protein function. However, despite the importance of this modification and the abundance of reports, all these data are scattered in the literature. No database/resource on methionine sulfoxidation exists currently. Since this information is useful to gain further insights into the redox regulation of cellular proteins, we have created a primary database of experimentally confirmed sulfoxidation sites.
MetOSite currently contains 7242 methionine sulfoxide sites found in 3562 different proteins from 23 species, with Homo sapiens, Arabidopsis thaliana and Bacillus cereus as the main contributors. Each collected site has been classified according to the effect of its sulfoxidation on the biological properties of the modified protein. Thus, MetOSite documents cases where the sulfoxidation of methionine leads to (i) gain of activity, (ii) loss of activity, (iii) increased protein-protein interaction susceptibility, (iv) decreased protein-protein interaction susceptibility, (v) changes in protein stability and (vi) changes in subcellular location.
MetOSite is available at https://metosite.uma.es.
蛋白质结合的蛋氨酸氧化形成蛋氨酸亚砜传统上被认为是一种氧化损伤。然而，越来越多的证据支持这种可逆反应也是一种调节性的翻译后修饰。因此，蛋氨酸残基的氧化已被报道对蛋白质功能有多种不同的影响。然而，尽管这种修饰很重要，而且有大量的报道，但所有这些数据都分散在文献中。目前没有关于蛋氨酸亚砜化的数据库/资源。由于这些信息对于深入了解细胞蛋白的氧化还原调节很有用，我们创建了一个实验证实的亚砜化位点的初级数据库。
MetOSite 目前包含 7242 个在 3562 种不同蛋白质中发现的蛋氨酸亚砜化位点，来自 23 个物种，其中人类、拟南芥和芽孢杆菌是主要贡献者。每个收集的位点都根据其亚砜化对修饰蛋白生物学特性的影响进行了分类。因此，MetOSite 记录了蛋氨酸亚砜化导致（i）活性增加，（ii）活性丧失，（iii）增加蛋白质-蛋白质相互作用易感性，（iv）降低蛋白质-蛋白质相互作用易感性，（v）蛋白质稳定性变化和（vi）亚细胞位置变化的情况。
MetOSite 可在 https://metosite.uma.es 上获取。
