Department of Biochemistry and Tissue Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, Brazil.
Biol Reprod. 2019 Aug 1;101(2):466-477. doi: 10.1093/biolre/ioz107.
Appropriate remodeling of the female lower reproductive tract and pelvic floor is essential during normal mammalian pregnancy, labor, and postpartum recovery. During mouse pregnancy, in addition to reproductive tract modifications, the pubic symphysis (PS) is remodeled into a soft interpubic ligament (IpL) to provide safe delivery of the offspring and fast postpartum recovery. Although temporal changes in the phenotypes of myeloid cells, such as mononuclear phagocytes, are crucial to remodeling the lower reproductive tract organs in preparation for a safe delivery, little is known about the involvement of recruited monocytes or macrophages in mouse PS remodeling. We used combined light microscopy, electron microscopy, and qPCR analysis to investigate the profile of recruited monocytes and macrophage polarization markers in C57Bl6 mouse interpubic tissues during pregnancy (D12, D18, and D19) and early days postpartum (1 dpp and 3 dpp) to better identify their presence in proper remodeling of the mouse PS. Our morphological data show that the number of recruited monocytes is increased in interpubic tissues and that recruited monocytes differentiate into proinflammatory or anti-inflammatory macrophage phenotypes from D18 to 3 dpp, which may contribute to dynamic changes in the gene expression of specific inflammatory mediators involved in interpubic tissue remodeling at these time points. Therefore, our morphological and quantitative gene expression data suggest that both differentiated macrophages from recruited monocytes and polarized macrophages may collaborate for IpL relaxation at labor and the appropriate repair of the PS after the first pregnancy.
在正常的哺乳动物妊娠、分娩和产后恢复期间，女性下生殖道和盆底的适当重塑是必不可少的。在小鼠妊娠期间，除了生殖道的改变，耻骨联合（PS）被重塑为柔软的耻骨间韧带（IpL），以确保胎儿的安全分娩和快速产后恢复。尽管单核细胞等髓样细胞表型的时间变化对于为安全分娩准备下生殖道器官的重塑至关重要，但招募的单核细胞或巨噬细胞在小鼠 PS 重塑中的参与知之甚少。我们使用组合的显微镜、电子显微镜和 qPCR 分析来研究 C57Bl6 小鼠耻骨间组织中招募的单核细胞和巨噬细胞极化标记物的特征，在妊娠期间（D12、D18 和 D19）和产后早期（1 dpp 和 3 dpp），以更好地识别它们在小鼠 PS 适当重塑中的存在。我们的形态学数据表明，招募的单核细胞数量在耻骨间组织中增加，并且从 D18 到 3 dpp，招募的单核细胞分化为促炎或抗炎的巨噬细胞表型，这可能有助于参与耻骨间组织重塑的特定炎症介质的基因表达在这些时间点发生动态变化。因此，我们的形态学和定量基因表达数据表明，来自招募的单核细胞的分化巨噬细胞和极化巨噬细胞可能在分娩时协同作用使 IpL 松弛，并在第一次怀孕后适当修复 PS。
