• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

由于环境的特定结构，导致变形虫状或间充质迁移表型的巨噬细胞的 RNA-seq。

RNA-seq of macrophages of amoeboid or mesenchymal migratory phenotype due to specific structure of environment.

机构信息

Department of Cell Biology, Charles University, Viničná 7, Prague, Czech Republic.

Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University (BIOCEV), Průmyslová 595, 25242, Vestec u Prahy, Czech Republic.

出版信息

Sci Data. 2018 Oct 2;5:180198. doi: 10.1038/sdata.2018.198.

DOI:10.1038/sdata.2018.198
PMID:30277482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6167950/
Abstract

M2-polarized macrophages have been shown to adapt their 3D migration mode to physical properties of surrounding extracellular matrix. They migrate in the integrin-mediated adhesion and proteolytic activity-dependent "mesenchymal" mode in stiff matrices and in the integrin and protease-independent "amoeboid" mode in low density, porous environments. To find out what impact the switching between the migration modes has on expression of both protein-coding and non-coding genes we employed RNA sequencing of total RNA depleted of ribosomal RNA isolated from macrophages migrating in either mode in 3D collagens. Differentially expressed genes from both categories have been detected and the changes in expression of selected genes were further validated with RT-qPCR. The acquired data will facilitate better understanding of how mechanical properties of tissue microenvironment reflect in macrophage immune function and how the transitions between mesenchymal and amoeboid migratory modes are regulated at the gene expression level.

摘要

M2 极化的巨噬细胞已经被证明能够根据周围细胞外基质的物理特性来调整其 3D 迁移模式。在刚性基质中，它们以整合素介导的黏附和蛋白水解活性依赖性的“间质”模式迁移，而在低密度多孔环境中，它们以整合素和蛋白酶非依赖性的“阿米巴样”模式迁移。为了了解迁移模式之间的转换对蛋白质编码基因和非编码基因表达的影响，我们采用了 RNA 测序技术，对在 3D 胶原中以两种模式迁移的巨噬细胞中分离的核糖体 RNA 去除后的总 RNA 进行了测序。从这两个类别中检测到了差异表达的基因，并通过 RT-qPCR 进一步验证了选定基因表达的变化。所获得的数据将有助于更好地理解组织微环境的机械特性如何反映在巨噬细胞免疫功能中，以及在基因表达水平上如何调节间质样和阿米巴样迁移模式之间的转变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d11/6167950/00066a8889f4/sdata2018198-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d11/6167950/75a3c67cb0b1/sdata2018198-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d11/6167950/dbd95ca0c51c/sdata2018198-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d11/6167950/00066a8889f4/sdata2018198-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d11/6167950/75a3c67cb0b1/sdata2018198-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d11/6167950/dbd95ca0c51c/sdata2018198-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d11/6167950/00066a8889f4/sdata2018198-f3.jpg

相似文献

1
RNA-seq of macrophages of amoeboid or mesenchymal migratory phenotype due to specific structure of environment.由于环境的特定结构，导致变形虫状或间充质迁移表型的巨噬细胞的 RNA-seq。
Sci Data. 2018 Oct 2;5:180198. doi: 10.1038/sdata.2018.198.
2
Matrix architecture dictates three-dimensional migration modes of human macrophages: differential involvement of proteases and podosome-like structures.基质架构决定了人类巨噬细胞的三维迁移模式：蛋白酶和似足突结构的差异参与。
J Immunol. 2010 Jan 15;184(2):1049-61. doi: 10.4049/jimmunol.0902223. Epub 2009 Dec 16.
3
Blood leukocytes and macrophages of various phenotypes have distinct abilities to form podosomes and to migrate in 3D environments.血液中的白细胞和各种表型的巨噬细胞具有不同的形成足突和在 3D 环境中迁移的能力。
Eur J Cell Biol. 2012 Nov-Dec;91(11-12):938-49. doi: 10.1016/j.ejcb.2012.07.002. Epub 2012 Sep 19.
4
Distinct Migratory Properties of M1, M2, and Resident Macrophages Are Regulated by αβ and αβ Integrin-Mediated Adhesion.M1、M2 和固有巨噬细胞的不同迁移特性受 αβ 和 αβ 整联蛋白介导的黏附调控。
Front Immunol. 2018 Nov 15;9:2650. doi: 10.3389/fimmu.2018.02650. eCollection 2018.
5
Control of macrophage 3D migration: a therapeutic challenge to limit tissue infiltration.控制巨噬细胞的三维迁移：限制组织浸润的治疗挑战。
Immunol Rev. 2014 Nov;262(1):216-31. doi: 10.1111/imr.12214.
6
Podosomes, But Not the Maturation Status, Determine the Protease-Dependent 3D Migration in Human Dendritic Cells.Podosomes 而非成熟状态决定人树突状细胞的蛋白酶依赖的 3D 迁移。
Front Immunol. 2018 Apr 30;9:846. doi: 10.3389/fimmu.2018.00846. eCollection 2018.
7
Viscoelastic gel-strip model for the simulation of migrating cells.用于模拟迁移细胞的黏弹性凝胶带模型。
Ann Biomed Eng. 2011 Nov;39(11):2735-49. doi: 10.1007/s10439-011-0360-z. Epub 2011 Jul 29.
8
Rho/ROCK pathway inhibition by the CDK inhibitor p27(kip1) participates in the onset of macrophage 3D-mesenchymal migration.细胞周期蛋白依赖性激酶抑制剂p27（kip1）对Rho/ROCK信号通路的抑制作用参与了巨噬细胞三维间充质迁移的起始过程。
J Cell Sci. 2014 Sep 15;127(Pt 18):4009-23. doi: 10.1242/jcs.150987. Epub 2014 Jul 11.
9
Material stiffness influences the polarization state, function and migration mode of macrophages.材料硬度会影响巨噬细胞的极化状态、功能和迁移方式。
Acta Biomater. 2019 Apr 15;89:47-59. doi: 10.1016/j.actbio.2019.02.048. Epub 2019 Feb 28.
10
Extracellular proteolysis in macrophage migration: losing grip for a breakthrough.细胞外蛋白水解在巨噬细胞迁移中的作用：为突破而放手。
Eur J Immunol. 2011 Oct;41(10):2805-13. doi: 10.1002/eji.201141538.

引用本文的文献

1
Adverse local tissue reactions in arthroplasty: opportunities and challenges for a common terminology across scientific, clinical and regulatory fields.关节成形术中的局部组织不良反应：科学、临床和监管领域通用术语面临的机遇与挑战
EFORT Open Rev. 2025 May 5;10(5):224-236. doi: 10.1530/EOR-2024-0116.
2
Microtubule-associated NAV3 regulates invasive phenotypes in glioblastoma cells.微管相关蛋白NAV3调节胶质母细胞瘤细胞的侵袭表型。
Brain Pathol. 2025 Jan;35(1):e13294. doi: 10.1111/bpa.13294. Epub 2024 Aug 3.
3
Targeted Single-cell Isolation of Spontaneously Escaping Live Melanoma Cells for Comparative Transcriptomics.

本文引用的文献

1
Confinement and low adhesion induce fast amoeboid migration of slow mesenchymal cells. confinement 和低粘附诱导缓慢间充质细胞的快速阿米巴样迁移。
Cell. 2015 Feb 12;160(4):659-672. doi: 10.1016/j.cell.2015.01.007.
2
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.使用DESeq2对RNA测序数据的倍数变化和离散度进行适度估计。
Genome Biol. 2014;15(12):550. doi: 10.1186/s13059-014-0550-8.
3
Physical influences of the extracellular environment on cell migration.细胞外环境对细胞迁移的物理影响。
靶向单细胞分离自发逃逸的活黑素瘤细胞进行比较转录组学分析。
Cancer Res Commun. 2023 Aug 11;3(8):1524-1537. doi: 10.1158/2767-9764.CRC-22-0305. eCollection 2023 Aug.
4
Multiscale model of the different modes of cancer cell invasion.多尺度模型研究癌细胞的不同侵袭模式。
Bioinformatics. 2023 Jun 1;39(6). doi: 10.1093/bioinformatics/btad374.
5
Amoeboid migration in health and disease: Immune responses cancer dissemination.健康与疾病中的阿米巴样迁移：免疫反应与癌症播散
Front Cell Dev Biol. 2023 Jan 5;10:1091801. doi: 10.3389/fcell.2022.1091801. eCollection 2022.
6
Histopathologic and Immunohistochemical Evaluation of Induced Lesions, Tissue Tropism and Host Responses following Experimental Infection of Egyptian Rousette Bats () with the Zoonotic Paramyxovirus, Sosuga Virus.埃及果蝠（）感染人畜共患病副黏病毒索萨加病毒后的诱导病变、组织嗜性和宿主反应的组织病理学和免疫组织化学评估。
Viruses. 2022 Jun 12;14(6):1278. doi: 10.3390/v14061278.
7
RNA-seq Characterization of Melanoma Phenotype Switch in 3D Collagen after p38 MAPK Inhibitor Treatment.p38MAPK 抑制剂处理后 3D 胶原中黑色素瘤表型转换的 RNA-seq 特征。
Biomolecules. 2021 Mar 17;11(3):449. doi: 10.3390/biom11030449.
8
Increased Level of Long Non-Coding RNA MALAT1 is a Common Feature of Amoeboid Invasion.长链非编码RNA MALAT1水平升高是阿米巴样侵袭的共同特征。
Cancers (Basel). 2020 May 1;12(5):1136. doi: 10.3390/cancers12051136.
9
Recruitment of monocytes and mature macrophages in mouse pubic symphysis relaxation during pregnancy and postpartum recovery†.妊娠和产后恢复期小鼠耻骨联合松弛过程中单核细胞和成熟巨噬细胞的募集。
Biol Reprod. 2019 Aug 1;101(2):466-477. doi: 10.1093/biolre/ioz107.
Nat Rev Mol Cell Biol. 2014 Dec;15(12):813-24. doi: 10.1038/nrm3897. Epub 2014 Oct 30.
4
Microenvironment and tumor cell plasticity: an easy way out.微环境与肿瘤细胞可塑性：一条轻而易举的出路。
Cancer Lett. 2013 Nov 28;341(1):80-96. doi: 10.1016/j.canlet.2013.01.042. Epub 2013 Jan 31.
5
STAR: ultrafast universal RNA-seq aligner.STAR：超快通用 RNA-seq 对齐工具。
Bioinformatics. 2013 Jan 1;29(1):15-21. doi: 10.1093/bioinformatics/bts635. Epub 2012 Oct 25.
6
Blood leukocytes and macrophages of various phenotypes have distinct abilities to form podosomes and to migrate in 3D environments.血液中的白细胞和各种表型的巨噬细胞具有不同的形成足突和在 3D 环境中迁移的能力。
Eur J Cell Biol. 2012 Nov-Dec;91(11-12):938-49. doi: 10.1016/j.ejcb.2012.07.002. Epub 2012 Sep 19.
7
NG2-mediated Rho activation promotes amoeboid invasiveness of cancer cells.NG2 介导的 Rho 激活促进癌细胞的阿米巴样浸润。
Eur J Cell Biol. 2012 Nov-Dec;91(11-12):969-77. doi: 10.1016/j.ejcb.2012.05.001. Epub 2012 Jun 13.
8
Turnover of focal adhesions and cancer cell migration.粘着斑的周转与癌细胞迁移
Int J Cell Biol. 2012;2012:310616. doi: 10.1155/2012/310616. Epub 2012 Jan 26.
9
Cancer invasion and the microenvironment: plasticity and reciprocity.癌症侵袭与微环境：可塑性与互为影响。
Cell. 2011 Nov 23;147(5):992-1009. doi: 10.1016/j.cell.2011.11.016.
10
Pigment epithelium-derived factor blocks tumor extravasation by suppressing amoeboid morphology and mesenchymal proteolysis.色素上皮衍生因子通过抑制阿米巴样形态和间质蛋白水解来阻止肿瘤渗出。
Neoplasia. 2011 Jul;13(7):633-42. doi: 10.1593/neo.11446.