噬菌体T4 RegA蛋白与信使核糖核酸（mRNA）结合并阻止翻译起始。
Bacteriophage T4 regA protein binds to mRNAs and prevents translation initiation.
作者信息
Winter R B, Morrissey L, Gauss P, Gold L, Hsu T, Karam J
机构信息
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
出版信息
Proc Natl Acad Sci U S A. 1987 Nov;84(22):7822-6. doi: 10.1073/pnas.84.22.7822.
The bacteriophage T4 regA protein is a translational repressor of a subset of phage mRNAs. We show here that purified regA protein binds specifically to target mRNAs near the initiating AUG and occludes binding of ribosomes. Translational repression by regA protein diminishes expression of many genes whose mRNA sequences around the initiating AUG codons are different. A comparison of nucleotide sequences from several regA-repressed mRNAs suggests that the initiating AUG is an important, but not sufficient, sequence for regA binding.
噬菌体T4的RegA蛋白是噬菌体部分mRNA的翻译阻遏物。我们在此表明，纯化的RegA蛋白特异性结合起始AUG附近的靶mRNA，并阻止核糖体的结合。RegA蛋白介导的翻译抑制减少了许多基因的表达，这些基因起始AUG密码子周围的mRNA序列各不相同。对几种受RegA抑制的mRNA的核苷酸序列比较表明，起始AUG是RegA结合的重要序列，但并非充分条件。
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