一种基于 PCR 的 NGS 方案，可直接从生物标本中对西尼罗河病毒 2 型进行全基因组测序。
A PCR-based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological specimens.
机构信息
Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.
National Reference Centre for Arboviruses, Department of Microbiology, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece.
出版信息
Mol Cell Probes. 2019 Aug;46:101412. doi: 10.1016/j.mcp.2019.06.002. Epub 2019 Jun 12.
Lineage 2 West Nile virus (WNV) strains have been implicated in severe encephalitis outbreaks in humans and equines residing in Europe. WNV molecular characterization is important for the development of diagnostic assays, as well as for obtaining molecular information, which is necessary for epidemiological investigations of virus transmission in areas at risk. For whole genome sequencing of lineage 2 WNV strains, directly from biological specimens, a PCR-based NGS protocol was developed. The method was applied in WNV-positive specimens obtained from animal, human and mosquito hosts in Greece. The results of its application indicate that, even in cases of low virus titers, the developed PCR-based NGS approach is able to provide whole genome sequences of lineage 2 WNV strains.
西尼罗病毒（WNV）谱系 2 株已被怀疑与欧洲人类和马属动物的严重脑炎疫情有关。WNV 的分子特征对于诊断检测的开发以及获得分子信息都很重要，这对于在有风险的地区进行病毒传播的流行病学调查是必要的。为了对来自生物样本的谱系 2 WNV 株进行全基因组测序，开发了一种基于 PCR 的 NGS 方案。该方法应用于从希腊的动物、人类和蚊子宿主中获得的WNV 阳性样本。该方法的应用结果表明，即使在病毒滴度较低的情况下，开发的基于 PCR 的 NGS 方法也能够提供谱系 2 WNV 株的全基因组序列。
Zotero 插件