The Main Arboviruses and Virus Detection Methods in Vectors: Current Approaches and Future Perspectives.

Arthropod-borne viruses (arboviruses) represent a growing concern for global public and veterinary health, with cases reported across all continents. This review presents a broad overview of the geographic distribution of arboviruses transmitted by insect vectors, emphasizing the importance of early viral detection as a cornerstone of surveillance and outbreak preparedness. Special attention is given to the phenomenon of zoonotic spillover, where viruses maintained in natural transmission cycles often involving wildlife reservoirs and arthropod vectors cross into human populations, triggering emergent or re-emergent outbreaks. This article discusses key arboviral families of medical and veterinary significance, including Togaviridae, Flaviviridae, Nairoviridae, Phenuiviridae, Peribunyaviridae, and Orthomyxoviridae, highlighting their molecular and structural characteristics. These features are essential for guiding the development and implementation of specific and sensitive detection strategies. In addition, this work provides a comparative analysis of diverse laboratory methodologies for viral detection in vectors. From serological assays and viral isolation to advanced molecular tools and next-generation sequencing, we explore their principles, practical applications, and context-dependent advantages and limitations. By compiling this information, we aim to support researchers and public health professionals in selecting the most appropriate tools for vector surveillance, ultimately contributing to improved response strategies in the face of arboviral threats.