Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, China.
Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, China.
Food Chem. 2019 Oct 30;296:40-46. doi: 10.1016/j.foodchem.2019.05.144. Epub 2019 May 22.
Gallotannin 1,2,6-tri-O-galloyl-β-d-glucopyranose (1,2,6-TGGP) plays multiple roles against multidrug-resistant bacteria and other diseases. Nevertheless, its availability in tea (Camellia sinensis) has rarely been reported. Herein, the identification and verification of 1,2,6-TGGP from Camellia sinensis using ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-qTOF MS/MS), electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) were reported. The isolated 1,2,6-TGGP was used for the chemotaxonomy analysis of 17 tea cultivars. The contents of 1,2,6-TGGP ranged from 1.96 to 43.20 mg g, with a mean of 13.75 mg g. Relatively high 1,2,6-TGGP contents (>30 mg g) in two tea cultivars indicate that the beneficial effects of 1,2,6-TGGP can be obtained by consuming these teas. The chemotaxonomy analysis showed a biosynthetic relation between 1,2,6-TGGP and gallic acid. Further analysis showed that the 1,2,6-TGGP contents significantly decreased with the plucking times irrespective of the cultivars. Moreover, a positive and significant correlation was also observed between 1,2,6-TGGP and gallic acid. The identification of tea cultivars that are rich in 1,2,6-TGGP was first reported and the obtained results should boost their potential use in food and medicine.
没食子酰基三葡萄糖（1,2,6-TGGP）在对抗多药耐药菌和其他疾病方面发挥着多种作用。然而，其在茶叶（Camellia sinensis）中的可用性却鲜有报道。本文采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-qTOF MS/MS）、电喷雾电离质谱（ESI-MS）和核磁共振（NMR）技术对茶叶中的 1,2,6-TGGP 进行了鉴定和验证。并利用分离得到的 1,2,6-TGGP 对 17 个茶树品种进行了化学分类分析。1,2,6-TGGP 的含量范围为 1.96-43.20mg/g，平均值为 13.75mg/g。两种茶树品种中 1,2,6-TGGP 含量较高（>30mg/g），这表明通过饮用这些茶可以获得 1,2,6-TGGP 的有益效果。化学分类分析表明 1,2,6-TGGP 与没食子酸之间存在生物合成关系。进一步的分析表明，1,2,6-TGGP 含量与采摘次数有关，且不受品种影响而显著降低。此外，1,2,6-TGGP 含量与没食子酸之间也存在显著的正相关关系。本文首次报道了富含 1,2,6-TGGP 的茶树品种的鉴定结果，这为其在食品和医药领域的潜在应用提供了依据。
