Wang Yueqi, Xun Hanshuo, Wang Liubin, Aktar Shirin, Lei Yuping, Zhang Rui, Wang Liyuan, Wei Kang
Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.
Foods. 2024 Feb 4;13(3):495. doi: 10.3390/foods13030495.
Hydrolyzed tannin 1,2,6-tri-O-galloyl-β-D-glucopyranose (1,2,6-TGGP) possesses significant medicinal properties. However, little is known about its underlying molecular mechanisms. In this study, the levels of 1,2,6-TGGP in tea materials from different cultivars and leaf positions were compared. Additionally, one leaf and one bud sample from six tea cultivars with significant variations in 1,2,6-TGGP levels were analyzed using transcriptome high-throughput sequencing to identify the genes that are responsible for 1,2,6-TGGP accumulation. The sequencing results were mapped to the reference tea genome, revealing a total of 2735 differentially expressed genes (DEGs). This set included four UDP glycosyltransferase (UGTs) and six serine carboxypeptidases-like (SCPLs) genes. Among them, the upregulated SCPLs () may directly participate in the acylation reaction of 1,2,6-TGGP. In addition, several classes of DEGs, including cytochrome P450, were significantly associated with the 1,2,6-TGGP content, which is potentially involved in their regulation. Overall, these results provide new insights into the molecular mechanism of 1,2,6-TGGP accumulation.
水解单宁1,2,6 - 三 - O - 没食子酰基 - β - D - 吡喃葡萄糖（1,2,6 - TGGP）具有显著的药用特性。然而，其潜在的分子机制却鲜为人知。在本研究中，比较了不同品种和叶位的茶叶原料中1,2,6 - TGGP的含量。此外，对1,2,6 - TGGP含量有显著差异的六个茶树品种的一芽一叶样本进行了转录组高通量测序分析，以鉴定负责1,2,6 - TGGP积累的基因。测序结果与参考茶树基因组进行比对，共鉴定出2735个差异表达基因（DEG）。其中包括四个尿苷二磷酸糖基转移酶（UGT）基因和六个类丝氨酸羧肽酶（SCPL）基因。其中，上调的SCPL基因可能直接参与1,2,6 - TGGP的酰化反应。此外，包括细胞色素P450在内的几类DEG与1,2,6 - TGGP含量显著相关，可能参与其调控。总体而言，这些结果为1,2,6 - TGGP积累的分子机制提供了新的见解。
