CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing, 100190, PR China.
CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing, 100190, PR China; Sino-Danish College, University of Chinese Academy of Sciences, Sino-Danish Center for Education and Research, Beijing, 100190, PR China.
Talanta. 2019 Oct 1;203:227-234. doi: 10.1016/j.talanta.2019.05.070. Epub 2019 May 21.
In this work, we reported a novel nanozyme synthesized by decorating highly dispersed ultrafine IrO nanoparticles on reduced graphene oxide (rGO) nanosheets via a simple hydrothermal method. The as-prepared IrO/rGO nanocomposites exhibited intrinsic peroxidase-like activity and could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce blue product in the presence of HO. Catalytic kinetic of IrO/rGO nanocomposites followed Michaelis-Menten behavior, exhibiting a higher affinity to TMB than horseradish peroxidase (HRP) enzyme. Catalytic mechanism studies suggested that the peroxidase-like activity of IrO/rGO nanocomposites originated from their ability of electron transfer between substrate and HO. On the basis of high peroxidase-like activity of IrO/rGO nanocomposites, a colorimetric strategy for rapid and sensitive detection of low weight biothiols was developed. The colorimetric detection assays for low weight biothiols showed high selectivity against other amino acids. Therefore, the IrO/rGO nanozyme is expected for promising potential applications in the biosensor, diagnostics and environment.
在这项工作中，我们通过简单的水热法报道了一种新型纳米酶，该纳米酶通过在还原氧化石墨烯（rGO）纳米片上修饰高度分散的超细 IrO 纳米颗粒而合成。所制备的 IrO/rGO 纳米复合材料表现出内在的过氧化物酶样活性，并在 HO 的存在下可以催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化生成蓝色产物。IrO/rGO 纳米复合材料的催化动力学遵循米氏行为，对 TMB 的亲和力高于辣根过氧化物酶（HRP）酶。催化机制研究表明，IrO/rGO 纳米复合材料的过氧化物酶样活性源于其在底物和 HO 之间进行电子转移的能力。基于 IrO/rGO 纳米复合材料的高过氧化物酶样活性，开发了一种用于快速灵敏检测低分子量生物硫醇的比色策略。用于检测低分子量生物硫醇的比色检测方法对其他氨基酸具有高选择性。因此，IrO/rGO 纳米酶有望在生物传感器、诊断和环境领域有很好的应用前景。
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