State Key Laboratory of Metastable Materials Science and Technology, Key Laboratory of Applied Chemistry, Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, Yanshan University, Qinhuangdao 066004, China.
Department of Molecular Chemistry and Materials Science, Weizmann Institute of Science, Rehovot 76100, Israel.
Biomolecules. 2021 Apr 28;11(5):647. doi: 10.3390/biom11050647.
Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with KPtCl. Pt-BGP NCs and catalyzed the decomposition of HO to generate •OH radicals, which could oxidize TMB to form oxidized TMB (oxTMB), indicating their peroxidase-like properties. The kinetics followed the Michaelis-Menten equation. Furthermore, the colorimetric detection of ascorbic acid using Pt-BGP NCs showed high selectivity and a low detection limit of 0.191 μM. The accuracy of real sample detection using Pt-BGP NCs was as high as 98.9%. More importantly, Pt-BGP NCs had high cell biocompatibility and extremely low hemolysis rate due to the component of BGP. In summary, the prepared Pt-BGP NCs with reductive activity and good biocompatibility have good application prospects in colorimetric detection of ascorbic acid.
抗坏血酸是一种具有抗氧化特性的有机化合物，可以保护人体免受自由基的威胁。因此，检测抗坏血酸的存在并测量其浓度以调节其在体内的含量非常重要。在这项工作中，我们通过将 BGP 与 KPtCl 反应制备了苦瓜多糖 (BGP)-稳定的铂纳米簇 (Pt-BGP NCs)。Pt-BGP NCs 可以催化 HO 的分解生成 •OH 自由基，这些自由基可以将 TMB 氧化形成氧化 TMB（oxTMB），表明其具有过氧化物酶样特性。动力学遵循米氏方程。此外，使用 Pt-BGP NCs 进行的抗坏血酸比色检测具有高选择性和低检测限，为 0.191 μM。使用 Pt-BGP NCs 进行的实际样品检测的准确性高达 98.9%。更重要的是，由于 BGP 的成分，Pt-BGP NCs 具有高细胞生物相容性和极低的溶血率。总之，具有还原活性和良好生物相容性的制备的 Pt-BGP NCs 在抗坏血酸的比色检测中具有良好的应用前景。
