Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Key Laboratory of Yellow River and Huai River Water Environment and Pollution Control, Ministry of Education, School of Environment, Henan Normal University, Xinxiang, Henan 453007, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Mar 15;269:120750. doi: 10.1016/j.saa.2021.120750. Epub 2021 Dec 13.
In this work, we reported a novel nanozyme (3D VO-MoS/rGO) by decorating MoS nano-flowers and VO nanoparticles on reduced graphene oxide (rGO). The 3D VO-MoS/rGO nanocomposites exhibited intrinsic peroxidase mimicking activity and catalyzed the oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB) to produce a blue colored product in the presence of HO. Compared with horseradish peroxidase (HRP), 3D VO-MoS/rGO nanocomposites displayed high catalytic velocity (V) and affinity (K) for substrates (HO and TMB). The study of the catalytic mechanism showed that the reduction of V and the oxidation of S in the 3D VO-MoS/rGO nanocomposites accelerate electron transfer between HO and TMB, which enhanced the peroxidase mimicking activity of 3D VO-MoS/rGO nanocomposites. The as-synthetized 3D VO-MoS/rGO could be used for the colorimetric detection of HO in the range of 20.00-800.00 μM with the LOD of 12.40 μM (3σ/S). Moreover, the 3D VO-MoS/rGO could also be used for the detection of glucose in the range of 4.00-300.00 μM with the LOD of 3.99 μM (3σ/S). In addition, the as-synthetized novel peroxidase mimics has good applicability for sensitive colorimetric determination of glucose in human blood samples and artificial urine samples, and has broad application prospects as a multi-functional sensing platform in clinical diagnosis.
在这项工作中，我们通过在还原氧化石墨烯（rGO）上修饰 MoS 纳米花和 VO 纳米颗粒，报道了一种新型纳米酶（3D VO-MoS/rGO）。3D VO-MoS/rGO 纳米复合材料表现出内在的过氧化物酶模拟活性，并在 HO 的存在下催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化，生成蓝色产物。与辣根过氧化物酶（HRP）相比，3D VO-MoS/rGO 纳米复合材料对底物（HO 和 TMB）具有更高的催化速度（V）和亲和力（K）。催化机制的研究表明，3D VO-MoS/rGO 纳米复合材料中 V 的还原和 S 的氧化加速了 HO 和 TMB 之间的电子转移，从而增强了 3D VO-MoS/rGO 纳米复合材料的过氧化物酶模拟活性。所合成的 3D VO-MoS/rGO 可用于 HO 在 20.00-800.00 μM 范围内的比色检测，LOD 为 12.40 μM（3σ/S）。此外，3D VO-MoS/rGO 还可用于检测 4.00-300.00 μM 范围内的葡萄糖，LOD 为 3.99 μM（3σ/S）。此外，所合成的新型过氧化物酶模拟物具有良好的适用性，可用于灵敏比色法测定人血样和人工尿样中的葡萄糖，作为一种多功能传感平台，在临床诊断中有广阔的应用前景。
