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乌鳢的转录组测序与分析

Transcriptome sequencing and analysis of the northern snakehead, .

作者信息

Xiao Mingsong, Bao Fangyin, Zhao Yan, Hu Qingsen

机构信息

College of Life and Health Science, Anhui Science and Technology University, Fengyang 233100, Anhui, People's Republic of China.

出版信息

J Genet. 2019 Jun;98(2).

Abstract

Northern snakehead, Cantor, is an endemic freshwater fish in China. However, wild stocks of are dwindling sharply. Further, water conservancy projects, environmental pollution and human activities have caused the decrease of wild stocks, which has attracted much attention. Here, we have investigated the genomic information of using IlluminaHiseq 4000 sequencing. The transcriptomes of were sequenced by Illumina technology. A total of 67,564 sequences from 79,500,964 paired-end reads were generated, 33,710 unigenes were annotated based on protein databases (NCBI nonredundant (NR) databases). In total, 7182 unigenes had the clusters of orthologous group (COG) classifications, 33,710 unigenes were assigned to 59 gene ontology (GO) terms. Further, a total of 21,464 simple sequence repeats (SSRs) from 67,564 unigenes and 113,518 single nucleotide polymorphism (SNP) sites among 335 Mclean reads were yielded for based on a transcriptome-wide search. The new transcriptome data which is presented in this study for will provide valuable information for gene discovery and downstream applications, such as phylogenetic analysis, gene-expression profiling and identification of genetic markers (SSRs andSNP).

摘要

乌鳢(Channa argus)是中国特有的淡水鱼。然而,其野生种群数量正在急剧减少。此外,水利工程、环境污染和人类活动导致了野生种群数量的下降,这一情况已引起广泛关注。在此,我们利用IlluminaHiseq 4000测序技术对乌鳢的基因组信息进行了研究。通过Illumina技术对乌鳢的转录组进行了测序。从79,500,964条双端读段中总共生成了67,564条序列,基于蛋白质数据库(NCBI非冗余(NR)数据库)对33,710条单基因进行了注释。总共7182条单基因具有直系同源簇(COG)分类,33,710条单基因被分配到59个基因本体(GO)术语中。此外,基于全转录组搜索,在乌鳢的67,564条单基因中总共产生了21,464个简单序列重复(SSR),在335M条clean读段中产生了113,518个单核苷酸多态性(SNP)位点。本研究中呈现的乌鳢新转录组数据将为基因发现和下游应用提供有价值的信息,如系统发育分析、基因表达谱分析和遗传标记(SSR和SNP)的鉴定。

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