Faculty of Medicine, Ain Shams University, Cairo, Egypt.
J Appl Microbiol. 2019 Sep;127(3):693-700. doi: 10.1111/jam.14347. Epub 2019 Jul 7.
Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt.
Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline, ciprofloxacin, erythromycin and clindamycin was investigated for 80 isolates of Staphylococci by BMD with the addition of dimethyl thiazole diphenyl tetrazolium bromide (MTT), agar dilution with the addition of MTT and triphenyl tetrazolium chloride at the standard bacterial concentration together with addition of MTT at an experimental bacterial concentration. BMD (MTT) showed the highest agreement in comparison with the standard BMD.
Colorimetric BMD was rapid and easy to interpret. Colorimetric agar dilution (MTT) was less tedious than BMD.
Colorimetric antibiotic susceptibility is a good option to provide rapid reliable results for critically ill patients. In addition, agar dilution (MTT) helps to investigate outbreaks of methicillin-resistant Staphylococcus aureus (MRSA), VISA or VRSA. BMD (MTT) can be performed routinely to detect VA MIC in MRSA blood stream infections and hospital acquired pneumonia, where, high VA MIC is associated with a higher mortality rate.
评估使用四唑盐肉汤微量稀释（BMD）和琼脂稀释方法对金黄色葡萄球菌进行抗菌药物敏感性试验的性能。
通过添加二甲基噻唑二苯基四氮唑溴盐（MTT）的 BMD、添加 MTT 的琼脂稀释以及在标准细菌浓度下添加 MTT 并在实验细菌浓度下添加 MTT 的方法，对 80 株金黄色葡萄球菌进行了八种抗菌药物（万古霉素（VA）、利奈唑胺、苯唑西林、庆大霉素（CN）、四环素、环丙沙星、红霉素和克林霉素）的最小抑菌浓度（MIC）研究。与标准 BMD 相比，BMD（MTT）显示出最高的一致性。
比色 BMD 快速且易于解释。比色琼脂稀释（MTT）比 BMD 繁琐程度低。
比色抗生素敏感性是为危重症患者提供快速可靠结果的不错选择。此外，琼脂稀释（MTT）有助于调查耐甲氧西林金黄色葡萄球菌（MRSA）、万古霉素中间金黄色葡萄球菌（VISA）或万古霉素耐药金黄色葡萄球菌（VRSA）的爆发。可以常规进行 BMD（MTT）以检测 MRSA 血流感染和医院获得性肺炎中的 VA MIC，其中高 VA MIC 与更高的死亡率相关。
