Park M S, Subbiah M T
Department of Internal Medicine, University of Cincinnati, Ohio.
Biochem Biophys Res Commun. 1987 Nov 30;149(1):208-12. doi: 10.1016/0006-291x(87)91625-1.
Previous analysis of amniotic fluid (AF) noted only the presence of high density lipoprotein (HDL). In this study AF lipoprotein profile was examined using gel filtration column chromatography and Ouchterlony gel diffusion. Unlike previous studies which showed only the presence of HDL, we found significant amounts of low density lipoprotein (LDL) and very low density lipoprotein (VLDL). AF-LDL and AF-VLDL were identified by reactions with anti-h-apolipoprotein AI and AII antiserum and anti-h-apolipoprotein B-antiserum, respectively. Furthermore, bulk of the cholesterol mass was carried in VLDL (53.6 +/- 7.7%) and LDL (32.5 +/- 4.3%) with minor amounts (13.9 +/- 1.3%) in HDL fraction. It is concluded that human AF contains all three lipoproteins with most of the cholesterol being carried in very low density lipoprotein fraction.
先前对羊水（AF）的分析仅发现了高密度脂蛋白（HDL）的存在。在本研究中，使用凝胶过滤柱色谱法和双向免疫扩散法检测了羊水脂蛋白谱。与先前仅显示存在HDL的研究不同，我们发现了大量的低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）。分别通过与抗人载脂蛋白AI和AII抗血清以及抗人载脂蛋白B抗血清反应鉴定出羊水低密度脂蛋白（AF-LDL）和羊水极低密度脂蛋白（AF-VLDL）。此外，大部分胆固醇存在于极低密度脂蛋白（53.6±7.7%）和低密度脂蛋白（32.5±4.3%）中，而高密度脂蛋白部分含量较少（13.9±1.3%）。结论是，人羊水含有所有三种脂蛋白，且大部分胆固醇存在于极低密度脂蛋白部分。
