Garber D W, Marsh J B
Department of Physiology and Biochemistry, Medical College of Pennsylvania, Philadelphia.
Biochim Biophys Acta. 1988 Apr 15;959(3):253-61. doi: 10.1016/0005-2760(88)90198-1.
Lipoprotein content and composition were studied in ascites fluid of puromycin aminonucleoside-nephrotic rats. All of the lipoprotein density classes were found in ascites fluid. Protein levels compared to plasma were: very low density lipoprotein (VLDL, d less than 1.006), 1.2%; intermediate density lipoprotein (IDL, 1.006 less than d less than 1.02), 2.6%; low density lipoprotein (LDL, 1.02 less than d less than 1.063), 1.0%; and high density lipoprotein (HDL, 1.063 less than d less than 1.21), 1.1%. The predominant protein in ascites fluid was albumin, present at 1.9% of the plasma level. Radioiodinated VLDL and HDL injected intravenously into nephrotic rats appeared in lipoprotein fractions of the ascites fluid. VLDL and IDL triacylglycerol content and particle diameter were low compared with plasma particles, suggesting peritoneal triacylglycerol lipase activity; such lipase activity could account for the increased proportion of LDL in the ascites fluid. Ascites fluid LDL and HDL phospholipid and free cholesterol were high and cholesteryl ester was low. Ascites lipoproteins contained the same apolipoproteins as plasma, but in different proportions. Ascites VLDL had higher apolipoprotein B and lower apolipoprotein E, while LDL and HDL had higher apolipoprotein E. Ascites HDL could be separated by heparin-Sepharose affinity column chromatography into a retained and a non-retained fraction, while nearly all nephrotic plasma HDL was non-retained. These data suggest that modification of ascites fluid lipoproteins occurs prior to their entry into the lymph and return to the blood, perhaps mediated by peritoneal macrophages.
对嘌呤霉素氨基核苷肾病大鼠腹水液中的脂蛋白含量和组成进行了研究。在腹水液中发现了所有脂蛋白密度类别。与血浆相比，蛋白质水平如下：极低密度脂蛋白（VLDL，d小于1.006），1.2%；中间密度脂蛋白（IDL，1.006小于d小于1.02），2.6%；低密度脂蛋白（LDL，1.02小于d小于1.063），1.0%；高密度脂蛋白（HDL，1.063小于d小于1.21），1.1%。腹水液中的主要蛋白质是白蛋白，其含量为血浆水平的1.9%。静脉注射到肾病大鼠体内的放射性碘化VLDL和HDL出现在腹水液的脂蛋白组分中。与血浆颗粒相比，VLDL和IDL的三酰甘油含量和颗粒直径较低，提示腹膜三酰甘油脂肪酶活性；这种脂肪酶活性可能是腹水液中LDL比例增加的原因。腹水液中的LDL和HDL磷脂及游离胆固醇含量高，而胆固醇酯含量低。腹水脂蛋白所含载脂蛋白与血浆相同，但比例不同。腹水VLDL的载脂蛋白B含量较高，载脂蛋白E含量较低，而LDL和HDL的载脂蛋白E含量较高。腹水HDL可通过肝素-琼脂糖亲和柱层析分离为保留组分和非保留组分，而几乎所有肾病血浆HDL都是非保留的。这些数据表明，腹水液脂蛋白在进入淋巴并返回血液之前就发生了修饰，可能是由腹膜巨噬细胞介导的。
