School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, China.
Jingzhou Academy of Agricultural Sciences, Jingzhou 434000, China.
Infect Genet Evol. 2019 Oct;74:103921. doi: 10.1016/j.meegid.2019.103921. Epub 2019 Jun 14.
The immune system of many invertebrates, including insects, has been shown to comprise memory, or specific immune priming. However, knowledge of the molecular mechanisms especially the candidate immune-related genes mediated the specificity of the immune priming are still very scarce and fragmentary. We therefore used two closely related Gram-negative pathogenic bacteria (Photorhabdus luminescens TT01 and P. luminescens H06) as the priming agents and employed Illumina/Solexa platform to investigate the transcriptional changes of the haemocytes of Bombyx mori larvae after priming.
In total, 23.0 Gbp of sequence data and 153,331,564 reads were generated, representing 10,496 genes. Approximately 89% of the genes or sequenced reads could be aligned to the silkworm reference genome. The differentially expressed genes (DEGs) of PBS-vs-TT01 (up-regulated expression of TT01 relative to PBS), PBS-vs-H06 (up-regulated expression of H06 relative to PBS) and TT01-vs-H06 (up-regulated expression of H06 relative to TT01) were 707, 159 and 461 respectively. In addition, expression patterns of 25 selected DEGs derived from quantitative real-time polymerase chain reaction (qRT-PCR) were consistent with their transcript abundance changes obtained by transcriptomic analyses. The DEGs are mainly related to pattern recognition receptors (PRRs), antimicrobial peptides (AMPs), signaling molecular, effector molecules, phagosome and spliceosome, indicating that they have participated in the regulation of the specific immune priming in the B. mori larvae.
The transcriptome profiling data sets from this study will provide valuable resources to better understand the molecular and biological mechanisms regulating the specificity of invertebrates' immune priming. All these will shed light on controlling insect pests or preventing epidemic of infectious diseases in economic invertebrates.
包括昆虫在内的许多无脊椎动物的免疫系统已被证明包括记忆或特定的免疫启动。然而，关于分子机制的知识，特别是介导免疫启动特异性的候选免疫相关基因，仍然非常稀缺和零碎。因此，我们使用两种密切相关的革兰氏阴性病原菌（发光杆菌 TT01 和 P. luminescens H06）作为启动剂，并采用 Illumina/Solexa 平台研究了家蚕幼虫血细胞在启动后的转录变化。
共产生了 23.0 Gbp 的序列数据和 153,331,564 个reads，代表 10,496 个基因。大约 89%的基因或测序reads 可以与家蚕参考基因组对齐。PBS-vs-TT01（相对于 PBS，TT01 的表达上调）、PBS-vs-H06（相对于 PBS，H06 的表达上调）和 TT01-vs-H06（相对于 TT01，H06 的表达上调）的差异表达基因（DEGs）分别为 707、159 和 461 个。此外，25 个定量实时聚合酶链反应（qRT-PCR）衍生的 DEG 的表达模式与转录组分析获得的转录丰度变化一致。DEGs 主要与模式识别受体（PRRs）、抗菌肽（AMPs）、信号分子、效应分子、吞噬体和剪接体有关，表明它们参与了家蚕幼虫特定免疫启动的调节。
本研究的转录组分析数据集将为更好地理解调节无脊椎动物免疫启动特异性的分子和生物学机制提供有价值的资源。所有这些都将为控制经济昆虫中的害虫或预防传染病的流行提供启示。
