Ge Wen-Jie, Hu Fei, Hong Ting-Ting, Thakur Kiran, Tang Shun-Ming, Zhang Jian-Guo, Wei Zhao-Jun
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China.
School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China.
Int J Mol Sci. 2025 Apr 4;26(7):3394. doi: 10.3390/ijms26073394.
This study explores the dual effects of selenium (Se) supplementation on silkworm development by analyzing miRNA expression profiles of fat bodies in silkworms under different Se concentrations (50 µM and 200 µM). Compared to the control, 84 miRNAs displayed different expression levels in the F_50 µM group, with 72 miRNAs up-regulated and 12 down-regulated; 152 miRNAs were differentially expressed in the F_200 µM group, with 124 up-regulated and 28 down-regulated. In the F_50 µM group, the target genes of differentially expressed miRNAs were mainly enriched in Toll and Imd signaling pathways, oxidative phosphorylation, and ribosome biogenesis in eukaryotes; however, mainly oxidative phosphorylation, ribosome biogenesis in eukaryotes, and the spliceosome were enriched in the F_200 µM group. Based on the results of the protein-protein interaction network and miRNA-target network, bmo-miR-2a-1-5p and bmo-miR-317-3p_L-2R+2 were screened as key miRNAs in the F_50 µM group and the F_200 µM group, respectively. The bmo-miR-2a-1-5p mainly targeted 10014128 (), 100862750 (), and 101744000 () genes, which were enriched in Toll and Imd signaling pathways. The bmo-miR-317-3p_L-2R+2 primarily regulated 101738508 () and 101746688 () genes, which were in the spliceosome pathway. Thus, our results demonstrated that Se supplementation improved the silkworm development via bmo-miR-2a-1-5p miRNA regulation of the Toll and Imd signaling pathways and inhibited it via bmo-miR-317-3p_L-2R+2 miRNA targeting the spliceosome pathway. Our data revealed that 50 µM Se supplementation could improve silkworm productivity; meanwhile, a 200 µM Se treatment displayed toxic effects, leading to impaired development.
本研究通过分析不同硒浓度（50μM和200μM）下家蚕脂肪体的miRNA表达谱，探讨了补充硒对家蚕发育的双重影响。与对照组相比，F_50μM组中有84个miRNA表达水平不同，其中72个miRNA上调，12个下调；F_200μM组中有152个miRNA差异表达，其中124个上调，28个下调。在F_50μM组中，差异表达miRNA的靶基因主要富集在Toll和Imd信号通路、氧化磷酸化以及真核生物中的核糖体生物合成；然而，F_200μM组中主要富集的是氧化磷酸化、真核生物中的核糖体生物合成以及剪接体。基于蛋白质-蛋白质相互作用网络和miRNA-靶标网络的结果，bmo-miR-2a-1-5p和bmo-miR-317-3p_L-2R+2分别被筛选为F_50μM组和F_200μM组中的关键miRNA。bmo-miR-2a-1-5p主要靶向10014128（）、100862750（）和101744000（）基因，这些基因富集在Toll和Imd信号通路中。bmo-miR-317-3p_L-2R+2主要调控101738508（）和101746688（）基因，这些基因位于剪接体通路中。因此，我们的结果表明，补充硒通过bmo-miR-2a-1-5p miRNA对Toll和Imd信号通路的调控促进了家蚕发育，并通过bmo-miR-317-3p_L-2R+2 miRNA靶向剪接体通路抑制了家蚕发育。我们的数据表明，补充50μM硒可以提高家蚕的生产力；同时，200μM硒处理显示出毒性作用，导致发育受损。
