Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Int Immunopharmacol. 2019 Sep;74:105689. doi: 10.1016/j.intimp.2019.105689. Epub 2019 Jun 14.
Neutrophils are short-lived cells of the innate immune system that have an important role in defending against pathogens by producing reactive oxygen species (ROS). Therefore, effective strategies for increasing neutrophil's viability and function may be beneficial, especially in many conditions such as infections and immunodeficiency diseases. Some studies suggest using multipotent mesenchymal stromal cells (MSCs) and MSC-conditioned media (MSC-CM) for this aim. But, there is no study on using MSC-derived exosomes for improving neutrophil's viability and function. So, we examined the effects of MSC-exosomes and also MSC-CM on neutrophil's function and survival and compared them with each other.
Exosomes and CM were isolated from human adipose tissue MSCs. Exosomes were characterized, and the protein content of them was determined. Neutrophils were isolated from five healthy donors, and the effects of the two independent treatments (exosomes and conditioned media) on neutrophil's apoptosis were measured by Annexin V-PI method, then neutrophil's function was evaluated using NBT and phagocytosis assays.
It was recognized that exosomes decreased neutrophils apoptosis and increased their phagocytosis capacity. The conditioned media augmented neutrophil's phagocytosis and reactive oxygen species (ROS) production, but it couldn't decrease neutrophil's apoptosis.
Briefly, we concluded that MSC-exosomes augment neutrophil's viability more than their function while MSC-CM increase neutrophil's function more than the survival. This report showed that the use of MSC-exosomes and CM might be useful for increasing immunity by improving neutrophil's function and survival.
中性粒细胞是先天免疫系统中寿命较短的细胞，通过产生活性氧物质 (ROS) 在抵御病原体方面发挥着重要作用。因此，增加中性粒细胞活力和功能的有效策略可能是有益的，特别是在感染和免疫缺陷等许多疾病中。一些研究表明，使用多能间充质基质细胞 (MSC) 和 MSC 条件培养基 (MSC-CM) 可以达到这一目的。但是，目前尚无关于使用 MSC 衍生的外泌体来改善中性粒细胞活力和功能的研究。因此，我们研究了 MSC 外泌体和 MSC-CM 对中性粒细胞活力和功能的影响，并将它们进行了比较。
从人脂肪组织 MSC 中分离出外泌体和 CM。对外泌体进行了表征，并测定了它们的蛋白含量。从 5 位健康供体中分离出中性粒细胞，通过 Annexin V-PI 法测量两种独立处理（外泌体和条件培养基）对中性粒细胞凋亡的影响，然后通过 NBT 和吞噬作用测定评估中性粒细胞的功能。
结果表明，外泌体可减少中性粒细胞凋亡并增加其吞噬能力。条件培养基增强了中性粒细胞的吞噬作用和活性氧物质 (ROS) 的产生，但不能减少中性粒细胞的凋亡。
总之，我们得出结论，MSC 外泌体增加中性粒细胞活力的作用强于其功能，而 MSC-CM 增加中性粒细胞功能的作用强于其活力。本报告表明，通过改善中性粒细胞的功能和活力，使用 MSC 外泌体和 CM 可能有助于增强免疫力。
