Comparison of the effects of adipose tissue mesenchymal stromal cell-derived exosomes with conditioned media on neutrophil function and apoptosis.

BACKGROUND

Neutrophils are short-lived cells of the innate immune system that have an important role in defending against pathogens by producing reactive oxygen species (ROS). Therefore, effective strategies for increasing neutrophil's viability and function may be beneficial, especially in many conditions such as infections and immunodeficiency diseases. Some studies suggest using multipotent mesenchymal stromal cells (MSCs) and MSC-conditioned media (MSC-CM) for this aim. But, there is no study on using MSC-derived exosomes for improving neutrophil's viability and function. So, we examined the effects of MSC-exosomes and also MSC-CM on neutrophil's function and survival and compared them with each other.

METHODS

Exosomes and CM were isolated from human adipose tissue MSCs. Exosomes were characterized, and the protein content of them was determined. Neutrophils were isolated from five healthy donors, and the effects of the two independent treatments (exosomes and conditioned media) on neutrophil's apoptosis were measured by Annexin V-PI method, then neutrophil's function was evaluated using NBT and phagocytosis assays.

RESULTS

It was recognized that exosomes decreased neutrophils apoptosis and increased their phagocytosis capacity. The conditioned media augmented neutrophil's phagocytosis and reactive oxygen species (ROS) production, but it couldn't decrease neutrophil's apoptosis.

DISCUSSION

Briefly, we concluded that MSC-exosomes augment neutrophil's viability more than their function while MSC-CM increase neutrophil's function more than the survival. This report showed that the use of MSC-exosomes and CM might be useful for increasing immunity by improving neutrophil's function and survival.