Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil.
Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasilia, DF, 70910-900, Brazil.
Virology. 2019 Aug;534:72-79. doi: 10.1016/j.virol.2019.06.002. Epub 2019 Jun 6.
An analysis of transcriptomes from the antennae of the three South American stink bugs (Euschistus heros, Chinavia ubica, and Dichelops melacanthus) revealed the presence of picorna-like virus genome-length RNAs with high sequence identity to the genome of Halyomorpha halys virus (HhV), originally discovered in the transcriptome of the brown marmorated stink bug, Halyomorpha halys. Features of the genome, phylogenetic relationships to other viruses, and the appearances of virus-like particles isolated from host stink bugs all confirm that these viruses are iflaviruses and isolates of an undescribed species. Iflavirus RNAs were present at high levels (40%-90% of transcriptome reads) in the stink bug antennal transcriptomes. In whole-insect transcriptomes of H. halys, HhV reads were >500-fold more abundant in adults than in nymphs. We identified from field population a subject of species E. heros infected by this iflavirus. The results of the analysis suggest that these iflaviruses are able to produce large quantities of their RNAs without causing any obvious pathology to their hosts.
对来自南美三个臭虫（Euschistus heros、Chinavia ubica 和 Dichelops melacanthus）触角的转录组进行分析，揭示了存在与棕褐异丽金龟病毒（Halyomorpha halys virus，HhV）基因组长度 RNA 具有高度序列同一性的 picorna-like 病毒，HhV 最初是在棕褐异丽金龟的转录组中发现的。基因组特征、与其他病毒的系统发育关系以及从宿主臭虫中分离出的病毒样颗粒的外观均证实这些病毒为 iflaviruses，是一种未被描述的新种。iflavirus RNA 在臭虫触角转录组中以高水平存在（占转录组读数的 40%-90%）。在棕褐异丽金龟的全昆虫转录组中，HhV 读码框在成虫中的丰度比若虫高 500 多倍。我们从野外种群中鉴定出一个被这种 iflavirus 感染的 E. heros 个体。分析结果表明，这些 iflaviruses 能够大量产生其 RNA，而不会对宿主造成任何明显的病理影响。
