Genome Biol Evol. 2020 Dec 6;12(12):2554-2560. doi: 10.1093/gbe/evaa195.
Busseola fusca (Fuller) (Lepidoptera: Noctuidae), the maize stalk borer, is a widespread crop pest in sub-Saharan Africa that has been the focus of biological research and intensive management strategies. Here, we present a comprehensive annotated transcriptome of B. fusca (originally collected in the Western Province of Kenya) based on ten pooled libraries including a wide array of developmental stages, tissue types, and exposures to parasitoid wasps. Parasitoid wasps have been used as a form of biocontrol to try and reduce crop losses with variable success, in part due to differential infectivities and immune responses among wasps and hosts. We identified a number of loci of interest for pest management, including genes potentially involved in chemoreception, immunity, and response to insecticides. The comprehensive sampling design used expands our current understanding of the transcriptome of this species and deepens the list of potential target genes for future crop loss mitigation, in addition to highlighting candidate loci for differential expression and functional genetic analyses in this important pest species.
黄脊长足虻（Busseola fusca（Fuller））（鳞翅目：夜蛾科）是撒哈拉以南非洲广泛存在的作物害虫，一直是生物研究和密集管理策略的重点。在这里，我们根据包括广泛发育阶段、组织类型和寄生蜂暴露在内的十个混合文库，提供了黄脊长足虻（最初在肯尼亚西部省采集）的综合注释转录组。寄生蜂已被用作生物防治的一种形式，试图减少作物损失，但成功率不一，部分原因是寄生蜂和宿主之间的感染性和免疫反应存在差异。我们确定了一些与害虫管理相关的感兴趣基因座，包括可能参与化感作用、免疫和对杀虫剂反应的基因。使用的综合采样设计扩展了我们对该物种转录组的现有认识，并加深了潜在目标基因的列表，以减轻未来作物损失，此外还突出了该重要害虫物种中差异表达和功能遗传分析的候选基因座。
